Microdetermination of cholesterol in serum lipoproteins

A two-step procedure for the microdetermination of cholesterol in serum lipoproteins is compared with cholesterol quantitation after density gradient ultracentrifugation. Serum lipoproteins from 10 mul of serum are separated by electrophoresis on agarose and visualized by precipitation with dextran...

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Bibliographic Details
Published in:Journal of lipid research 1977-03, Vol.18 (2), p.259-263
Main Authors: Heuck, C C, Nothhelfer, A, Raetzer, H, Schlierf, G
Format: Article
Language:English
Subjects:
Cholesterol - analogs & derivatives
Cholesterol Esters - blood
Chromatography, Gas - methods
Colorimetry - methods
Evaluation Studies as Topic
gasliquid chromatography
Humans
Hyperlipidemias - blood
lipoprotein electrophoresis
Lipoproteins - blood
Lipoproteins, HDL - blood
Lipoproteins, LDL - blood
Lipoproteins, VLDL - blood
Microchemistry
Spectrophotometry - methods
Ultracentrifugation - methods
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Summary:A two-step procedure for the microdetermination of cholesterol in serum lipoproteins is compared with cholesterol quantitation after density gradient ultracentrifugation. Serum lipoproteins from 10 mul of serum are separated by electrophoresis on agarose and visualized by precipitation with dextran sulfate--CaCl2. The lipoprotein bands are cut off from the plates, the agarose slices are hydrolyzed by gas-liquid chromatography. The comparison between the two procedures reveals satisfactory correlations for beta-and pre-beta-lipoproteins and total serum. There is excellent recovery of cholesterol in fractionated lipoproteins.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)41705-5