Expression of M30 and M65 in celiac disease. Analytical cross-sectional study

• Published in: São Paulo medical journal 2018-11, Vol.136 (6), p.525-532
• Main Authors: Aksoy, Evrim Kahramanoğlu, Şimşek, Gülçin Güler, Torgutalp, Murat, Sapmaz, Ferdane Pirinççi, Akpınar, Muhammet Yener, Uzman, Metin, Nazlıgül, Yaşar
• Format: Article
• Language: English
• Subjects: Adult; Apoptosis; Biomarkers - metabolism; Biopsy; Caspases - metabolism; Celiac disease; Celiac Disease - diagnosis; Celiac Disease - metabolism; Celiac Disease - pathology; Cross-Sectional Studies; Female; Humans; Keratin-18 - metabolism; M30 cytokeratin-18 peptide, human; M65 antigen, human; Male; MEDICINE, GENERAL & INTERNAL; Middle Aged
• ISSN: 1516-3180; 1806-9460; 1806-9460
• DOI: 10.1590/1516-3180.2018.0241161118

The role of villous atrophy in apoptosis, a distinctive feature of celiac disease, is a matter of controversy. The aim of this study was to determine the apoptosis rate through immunohistochemical staining for M30 and M65 in celiac disease cases. Analytical cross-sectional study in a tertiary-level center. Duodenal biopsies from 28 treatment-naive patients with celiac disease, 16 patients with potential celiac disease, 10 patients with a gluten-free diet and 8 controls were subjected to immunohistochemical staining for the end-apoptotic marker M30 and the total cell death marker M65. H-scores were compared. Several laboratory parameters were recorded concomitantly, and at the one-year follow-up for celiac disease and potential celiac disease patients. There was a significant difference in H-score for M30 expression between the celiac disease, potential celiac disease and gluten-free diet groups (P = 0.009). There was no significant difference in H-score for M65 expression. There was a positive correlation between the H-score for M30 expression and the anti-tissue transglutaminase immunoglobulin A (anti-tTgIgA) and anti-tissue transglutaminase immunoglobulin G (anti-tTgIgG) levels (R = 0.285, P = 0.036; and R = 0.307, P = 0.024, respectively); and between the H-score for M65 expression and the anti-tTgIgA and anti-tTgIgG levels (R = 0.265, P = 0.053; and R=0.314, P = 0.021, respectively). There was no difference between celiac disease and potential celiac disease patients regarding the laboratory parameters selected. The rates of apoptosis and nutritional deficiencies in patients with potential celiac disease were similar to those in patients with celiac disease.