Distinct Transcriptional Profile of PDZ Genes after Activation of Human Macrophages and Dendritic Cells

The PDZ (PSD95, Dlg and ZO-1) genes encode proteins that primarily function as scaffolds of diverse signaling pathways. To date, 153 PDZ genes have been identified in the human genome, most of which have multiple protein isoforms widely studied in epithelial and neural cells. However, their expressi...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-07, Vol.23 (13), p.7010
Main Authors: Rosas-García, Jorge, Ramón-Luing, Lucero A., Bobadilla, Karen, Meraz-Ríos, Marco Antonio, Sevilla-Reyes, Edgar E., Santos-Mendoza, Teresa
Format: Article
Language:English
Subjects:
Antigen-presenting cells
Antigens
Cell activation
Cluster analysis
Dendritic cells
Gene expression
Genes
Genomes
Immune system
Influenza
Isoforms
Macrophages
Pathogens
PDZ
Phagocytes
Postsynaptic density proteins
Proteins
scaffold usage
Scaffolds
Signal transduction
Stimulation
Transcription
Transcription activation
transcriptional signature
Zonula occludens-1 protein
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Summary:The PDZ (PSD95, Dlg and ZO-1) genes encode proteins that primarily function as scaffolds of diverse signaling pathways. To date, 153 PDZ genes have been identified in the human genome, most of which have multiple protein isoforms widely studied in epithelial and neural cells. However, their expression and function in immune cells have been poorly studied. Herein, we aimed to assess the transcriptional profiles of 83 PDZ genes in human macrophages (Mɸ) and dendritic cells (DCs) and changes in their relative expression during cell PRR stimulation. Significantly distinct PDZ gene transcriptional profiles were identified under different stimulation conditions. Furthermore, a distinct PDZ gene transcriptional signature was found in Mɸ and DCs under the same phagocytic stimuli. Notably, more than 40 PDZ genes had significant changes in expression, with potentially relevant functions in antigen-presenting cells (APCs). Given that several PDZ proteins are targeted by viral products, our results support that many of these proteins might be viral targets in APCs as part of evasion mechanisms. Our results suggest a distinct requirement for PDZ scaffolds in Mɸ and DCs signaling pathways activation. More assessments on the functions of PDZ proteins in APCs and their role in immune evasion mechanisms are needed.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23137010