Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy

The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. The...

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Bibliographic Details
Published in:Photosynthetica 2023-01, Vol.61 (4), p.483-491
Main Authors: Kaňa, R, Šedivá, B, Prášil, O
Format: Article
Language:English
Subjects:
airyscan
cyanobacteria
frap
microdomain
photosystem
protein mobility
super-resolution microscopy
thylakoid membrane heterogeneity
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Summary:The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using super-resolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes; countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions.
ISSN:0300-3604
1573-9058
1573-9058
DOI:10.32615/ps.2023.043