Nitrogen Addition and Heterotroph Exclusion Affected Plant Species Diversity-Biomass Relationship by Affecting Plant Functional Traits

• Published in: Plants (Basel) 2024-01, Vol.13 (2), p.258
• Main Authors: Xu, Xile, Yang, Luping, Shen, Kai, Cao, Huijuan, Lin, Yishi, Liu, Jinliang, Han, Wenjuan
• Format: Article
• Language: English
• Subjects: Agricultural production; Area; Arthropods; Biodiversity; Biomass; Biomass energy; Comparative analysis; Ecological function; Environmental aspects; Flowers & plants; functional traits; Fungi; Fungicides; heterotroph removal; Heterotrophs; Insecticides; Leaf area; Leaves; Measurement; Nitrogen; nitrogen addition; Nitrogen in the body; Pathogens; Pesticides; Physiological aspects; Plant biomass; Plant diversity; Plant species; Plant-animal interactions; Plants; Plants (botany); Productivity; Species diversity; species identity; Species richness; Trophic levels; Understory
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13020258

(1) Background: Heterotrophs can affect plant biomass and alter species diversity-productivity relationships. However, these studies were conducted in systems with a low nitrogen (N) availability, and it is unclear how heterotroph removal affects the relationship between plant species diversity and productivity in different N habitats. (2) Methods: Three typical understory herbaceous plants were selected to assemble the plant species diversity (three plant species richness levels (1, 2, and 3) and seven plant species compositions), and the control, insecticide, fungicide, and all removal treatments were performed at each plant species diversity level in systems with or without N addition treatments. (3) Results: In systems without N addition, the insecticide treatment increased the plant aboveground biomass, total biomass, and leaf area, while the fungicide treatment reduced the plant belowground biomass, root length, and root tip number; the presence of increased the plant aboveground biomass. Similarly, the presence of increased the plant belowground biomass and root diameter under each heterotroph removal treatment. In systems with N addition, all removal treatments reduced the plant belowground biomass and increased the plant leaf area; the presence of significantly increased the plant aboveground biomass, total biomass, and root length under each heterotroph removal treatment. The presence of significantly increased the plant belowground biomass and leaf area under insecticide and fungicide treatments. (4) Conclusions: Heterotroph removal alters the plant species diversity-biomass relationship by affecting the plant functional traits in systems with different N availabilities. The impact of biodiversity at different trophic levels on ecosystem functioning should be considered under the background of global change.