Influence of the application of enemas with oily extract of curcumin (Curcuma longa) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream

• Published in: Journal of Coloproctology 2017-12, Vol.37 (4), p.312-319
• Main Authors: Martinez, Carlos Augusto Real, Oliveira, Vitor Piquera de, Campos, Fabio Guilherme, Kanno, Danilo Toshio, Fernandez, Oscar Orlando Araya, Kadri, Caled Jaoudat, Pereira, José Aires
• Format: Article
• Language: English
• Subjects: Análise de imagem assistida por computador; Colite; Colitis; Fatty acids, volatile; GASTROENTEROLOGY & HEPATOLOGY; Image processing, computer-assisted; Mucinas; Mucins; Original Article; SURGERY; Ácidos graxos de cadeia curta, voláteis
• ISSN: 2237-9363; 2317-6423; 2317-6423
• DOI: 10.1016/j.jcol.2017.08.002

To verify if the application of enemas containing oily extracts of curcumin preserves the tissue content of mucins in the glands of the colonic mucosa without fecal stream. Thirty-six Wistar rats were submitted to diversion of the fecal stream by proximal colostomy and distal mucous fistula. The animals were subdivided into three groups, and accordingly received enemas with saline and oily extract of curcumin at concentrations of 50mg/kg/day or 200mg/kg/day. After two or four weeks of intervention, the irrigated colic segments were removed. Neutral and acidic mucins were identified by Periodic-acid Schiff and Alcian-Blue techniques, respectively. The content of both mucin subtypes was measured by computerized morphometry. Mann–Whitney test was used to analyze the results, adopting a significance level of 5% (p≤0.05). There was an increase in the tissue content of neutral mucins in animals treated with curcumin at a concentration of 50mg/kg/day for four weeks, whereas in the group treated with 200mg/kg/day there was an increase independent of the time of intervention. The content of acidic mucins increased in animals treated with 200mg/kg/day regardless of the intervention time, whereas in those treated with 50mg/kg/day an increase was observed only after four weeks. Enemas with curcumin preserve the content of neutral and acidic mucins in the colonic epithelium without fecal stream. Verificar se a aplicação de clisteres com extrato oleoso de curcumina preserva o conteúdo de mucinas nas glândulas da mucosa cólica sem trânsito intestinal. Trinta e seis ratos Wistar foram submetidos à derivação intestinal por colostomia proximal e fístula mucosa distal. Os animais foram subdivididos em três grupos, segundo receberem clisteres com soro fisiológico 0,9%, extrato oleoso de curcumina nas concentrações de 50mg/kg/dia ou 200mg/kg/dia. Após duas ou quatro semanas de intervenção foram removidos os segmentos cólicos irrigados. As mucinas neutras e ácidas foram identificadas pelas técnicas do PAS e Alcian-Blue, respectivamente. O conteúdo tecidual de ambos os subtipos de mucinas foi mensurado por morfometria computadorizada. Utilizou-se teste de Mann-Whitney para análise dos resultados adotando-se nível de significância de 5% (p≤0,05). Houve aumento no conteúdo de mucinas neutras nos animais tratados com curcumina na concentração de 50mg/kg/dia por quatro semanas, enquanto nos tratados com 200mg/kg/dia houve aumento independente do tempo de intervenção. O conteúdo de m