Development of Fluorescence-Based Assays for Key Viral Proteins in the SARS-CoV-2 Infection Process and Lifecycle

Since the appearance of SARS-CoV-2 in 2019, the ensuing COVID-19 (Corona Virus Disease 2019) pandemic has posed a significant threat to the global public health system, human health, life, and economic well-being. Researchers worldwide have devoted considerable efforts to curb its spread and develop...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2024-03, Vol.25 (5), p.2850
Main Authors: Deng, Mingzhenlong, Zhang, Chuang, Yan, Wanli, Chen, Lei, He, Bin, Li, Yan
Format: Article
Language:English
Subjects:
antiviral agents
Antiviral Agents - therapeutic use
assay development
Computer simulation
COVID-19
Disease transmission
Drugs
Epidemics
Fluorescence
Genomes
Health aspects
Humans
infection
Infections
Medical research
Methods
Middle East respiratory syndrome
Peptide Hydrolases
Pneumonia
Proteases
Proteins
Respiratory diseases
Review
RNA
RNA polymerase
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Viral infections
Viral Proteins
Viruses
Well being
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Since the appearance of SARS-CoV-2 in 2019, the ensuing COVID-19 (Corona Virus Disease 2019) pandemic has posed a significant threat to the global public health system, human health, life, and economic well-being. Researchers worldwide have devoted considerable efforts to curb its spread and development. The latest studies have identified five viral proteins, spike protein (Spike), viral main protease (3CLpro), papain-like protease (PLpro), RNA-dependent RNA polymerase (RdRp), and viral helicase (Helicase), which play crucial roles in the invasion of SARS-CoV-2 into the human body and its lifecycle. The development of novel anti-SARS-CoV-2 drugs targeting these five viral proteins holds immense promise. Therefore, the development of efficient, high-throughput screening methodologies specifically designed for these viral proteins is of utmost importance. Currently, a plethora of screening techniques exists, with fluorescence-based assays emerging as predominant contenders. In this review, we elucidate the foundational principles and methodologies underpinning fluorescence-based screening approaches directed at these pivotal viral targets, hoping to guide researchers in the judicious selection and refinement of screening strategies, thereby facilitating the discovery and development of lead compounds for anti-SARS-CoV-2 pharmaceuticals.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25052850