EsxA, a type VII secretion system-dependent effector, reveals a novel function in the sporulation of Bacillus cereus ATCC14579

• Published in: BMC microbiology 2024-09, Vol.24 (1), p.351-15, Article 351
• Main Authors: Kamboyi, Harvey K, Paudel, Atmika, Shawa, Misheck, Sugawara, Misa, Zorigt, Tuvshinzaya, Chizimu, Joseph Y, Kitao, Tomoe, Furuta, Yoshikazu, Hang'ombe, Bernard M, Munyeme, Musso, Higashi, Hideaki
• Format: Article
• Language: English
• Subjects: Analysis; Bacillus cereus; Bacillus cereus - genetics; Bacillus cereus - growth & development; Bacillus cereus - metabolism; Bacillus cereus - physiology; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Cell culture; Chromatography; Chromatography, Liquid; Clonal deletion; Control; Deletion; E coli; Effectors; Endonuclease; EsxA; Food poisoning; Gene deletion; Gene mutations; Genes; Genetic aspects; Gram-positive bacteria; Health aspects; Homing endonuclease; Homologous recombination; Homology; Identification and classification; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Mutants; Mutation; Peptides; Plasmids; Point mutation; Prevention; Proteins; Risk factors; Scientific imaging; Secretion; Spores; Spores, Bacterial - genetics; Spores, Bacterial - growth & development; Spores, Bacterial - metabolism; Sporulation; Strain analysis; Substrates; Tandem Mass Spectrometry; Type VII Secretion Systems - genetics; Type VII Secretion Systems - metabolism; Type VIIb secretion system; Virulence
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-024-03492-1

Bacillus cereus is a Gram-positive, spore-forming bacterium that produces a spectrum of effectors integral to bacterial niche adaptation and the development of various infections. Among those is EsxA, whose secretion depends on the EssC component of the type VII secretion system (T7SS). EsxA's roles within the bacterial cell are poorly understood, although postulations indicate that it may be involved in sporulation. However, the T7SS repertoire in B. cereus has not been reported, and its functions are unestablished. We used the type strain, B. cereus ATCC14579, to generate ΔessC mutant through homologous recombination using the homing endonuclease I-SceI mediated markerless gene replacement. Comparatively, we analyzed the culture supernatant of type strain and the ΔessC mutant through Liquid chromatography-tandem mass spectrometry (LC-MS/MS). We further generated T7SSb-specific gene mutations to explore the housekeeping roles of the T7SSb-dependent effectors. The sporulation process of B. cereus ATCC14579 and its mutants was observed microscopically through the classic Schaeffer-Fulton staining method. The spore viability of each strain in this study was established by enumerating the colony-forming units on LB agar. Through LC-MS/MS, we identified a pair of nearly identical (94%) effector proteins named EsxA belonging to the sagEsxA-like subfamily of the WXG100 protein superfamily in the culture supernatant of the wild type and none in the ΔessC mutant. Homology analysis of the T7SSb gene cluster among B. cereus strains revealed diversity from the 3' end of essC, encoding additional substrates. Deletions in esxA1 and esxA2 neither altered cellular morphology nor growth rate, but the ΔesxA1ΔesxA2 deletion resulted in significantly fewer viable spores and an overall slower sporulation process. Within 24 h culture, more than 80% of wild-type cells formed endospores compared to less than 5% in the ΔesxA1ΔesxA2 mutant. The maximum spore ratios for the wild type and ΔesxA1ΔesxA2 were 0.96 and 0.72, respectively. Altogether, these results indicated that EsxA1 and EsxA2 work cooperatively and are required for sporulation in B. cereus ATCC14567. B. cereus ATCC14579 possesses two nearly identical T7SSb-dependent effectors belonging to the sagEsxA-like proteins. Simultaneous deletion of genes encoding these effectors significantly delayed and reduced sporulation, a novel finding for EsxA.