Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig

Despite playing a key role in digestion, there is only a broad characterization of the spatiotemporal development of the three glandular regions of the stomach (cardiac, fundic and pyloric) in the weaned pig. Hence, the objective of this experiment was to explore the differential expression (DE) of...

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Bibliographic Details
Published in:Veterinary sciences 2023-07, Vol.10 (7), p.473
Main Authors: Kiernan, Dillon P, O'Doherty, John V, Connolly, Kathryn Ruth, Ryan, Marion, Sweeney, Torres
Format: Article
Language:English
Subjects:
acid secretion
Acids
Animal experimentation
Aquaporin 4
cardiac gland region
cardiac-to-oxyntic transition
Diarrhea
Digestive enzymes
Enzymes
Feeds
fundic gland region
Gene expression
Gene regulation
Genes
Genetic research
Glands
Health aspects
Heart
Hogs
KCNQ1 protein
Lactose
monogastric
Pathogens
Pork industry
Potassium channels (voltage-gated)
pyloric gland region
Software
Statistical analysis
Stomach
Swine
Weaning
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Summary:Despite playing a key role in digestion, there is only a broad characterization of the spatiotemporal development of the three glandular regions of the stomach (cardiac, fundic and pyloric) in the weaned pig. Hence, the objective of this experiment was to explore the differential expression (DE) of a panel of key genes within the three glandular regions of the stomach. Eight pigs were sacrificed at d 8 post-weaning, and three mucosal samples were collected from each stomach's glandular regions. The expression of a panel of genes were measured using QPCR. The true cardiac gland region was characterized by increased expression of , , and relative to the two other regions ( < 0.05). The fundic gland region was characterized by increased expression of , C , , , , , , , , and compared to the two other regions ( < 0.05). The pyloric gland region was characterized by exclusive expression of ( < 0.05). A transition region between the cardiac and fundic region (cardiac-to-oxyntic transition) was observed with a gene expression signature that resembles a cross of the signatures found in the two regions. In conclusion, unique gene expression signatures were identifiable in each of the glandular regions, with a cardiac-to-oxyntic transition region clearly identifiable in the post-weaned pigs' stomachs.
ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci10070473