Alterations in Genome Organization in Lymphoma Cell Nuclei due to the Presence of the t(14;18) Translocation

Chromosomal rearrangements have been shown to alter genome organization, consequently having an impact on gene expression. Studies on certain types of leukemia have shown that gene expression can be exacerbated by the altered nuclear positioning of fusion genes arising from chromosomal translocation...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-02, Vol.25 (4), p.2377
Main Authors: Garimberti, Elisa, Federico, Concetta, Ragusa, Denise, Bruno, Francesca, Saccone, Salvatore, Bridger, Joanna Mary, Tosi, Sabrina
Format: Article
Language:English
Subjects:
18
BCL2
Cancer
Cell Nucleus - genetics
Chi-square test
chromosomal translocation
Chromosomes
Ewings sarcoma
Gene expression
Genes
Genetic aspects
Genomes
Genomics
Humans
IGH
In Situ Hybridization, Fluorescence
Leukemia
Localization
Lymphocytes
Lymphoma
Lymphoma - genetics
Non-Hodgkin's lymphomas
Proto-Oncogene Proteins c-bcl-2 - genetics
Translocation, Genetic
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Summary:Chromosomal rearrangements have been shown to alter genome organization, consequently having an impact on gene expression. Studies on certain types of leukemia have shown that gene expression can be exacerbated by the altered nuclear positioning of fusion genes arising from chromosomal translocations. However, studies on lymphoma have been, so far, very limited. The scope of this study was to explore genome organization in lymphoma cells carrying the t(14;18)(q32;q21) rearrangement known to results in over-expression of the gene. In order to achieve this aim, we used fluorescence in situ hybridization to carefully map the positioning of whole chromosome territories and individual genes involved in translocation in the lymphoma-derived cell line Pfeiffer. Our data show that, although there is no obvious alteration in the positioning of the whole chromosome territories, the translocated genes may take the nuclear positioning of either of the wild-type genes. Furthermore, the gene was looping out in a proportion of nuclei with the t(14;18) translocation but not in control nuclei without the translocation, indicating that chromosome looping may be an essential mechanism for expression in lymphoma cells.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25042377