In vitro and in vivo evaluation of virus-induced innate immunity in mouse

• Published in: STAR protocols 2021-09, Vol.2 (3), p.100708-100708, Article 100708
• Main Authors: Shen, Long, Shan, Xiao, Hu, Penghui, Zhang, Yanan, Ji, Zemin, Ni, Lina, Yuan, Yukang, Zheng, Hui, Wang, Ting, Yu, Qiujing
• Format: Article
• Language: English
• Subjects: Cell-based Assays; Immunology; Metabolism; Microbiology; Model Organisms; Protocol
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2021.100708

Innate immunity is the first line of host defense against viral infection. As one of the innate immune cell types, antigen-presenting cells play an important role in the process of antiviral immunity. This protocol describes the analysis of innate immunity induced by vesicular stomatitis virus infection of peritoneal macrophages in vitro and in vivo detection of IFN-β production and lung injury. For complete details on the use and execution of this protocol, please refer to Shen et al. (2021). [Display omitted] •Detailed protocol for VSV virus amplification and titer detection•Fast and efficient collection of peritoneal macrophages from mice•Analysis of in vitro innate immunity induced by VSV infection of peritoneal macrophages•In vivo system to model VSV infection induced IFN-β production and lung injury Innate immunity is the first line of host defense against viral infection. As one of the innate immune cell types, antigen-presenting cells play an important role in the process of antiviral immunity. This protocol describes the analysis of innate immunity induced by vesicular stomatitis virus infection of peritoneal macrophages in vitro and in vivo detection of IFN-β production and lung injury.