Modeling inherited retinal diseases using human induced pluripotent stem cell derived photoreceptor cells and retinal pigment epithelial cells

Since the discovery of induced pluripotent stem cell (iPSC) technology, there have been many attempts to create cellular models of inherited retinal diseases (IRDs) for investigation of pathogenic processes to facilitate target discovery and validation activities. Consistency remains key in determin...

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Bibliographic Details
Published in:Frontiers in medicine 2024-07, Vol.11, p.1328474
Main Authors: Seah, Ivan, Goh, Debbie, Banerjee, Animesh, Su, Xinyi
Format: Article
Language:English
Subjects:
induced pluripotent stem cell
inherited retinal diseases
Medicine
organoids
photoreceptor cells
retinal pigment epithelium
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Summary:Since the discovery of induced pluripotent stem cell (iPSC) technology, there have been many attempts to create cellular models of inherited retinal diseases (IRDs) for investigation of pathogenic processes to facilitate target discovery and validation activities. Consistency remains key in determining the utility of these findings. Despite the importance of consistency, quality control metrics are still not widely used. In this review, a toolkit for harnessing iPSC technology to generate photoreceptor, retinal pigment epithelial cell, and organoid disease models is provided. Considerations while developing iPSC-derived IRD models such as iPSC origin, reprogramming methods, quality control metrics, control strategies, and differentiation protocols are discussed. Various iPSC IRD models are dissected and the scientific hurdles of iPSC-based disease modeling are discussed to provide an overview of current methods and future directions in this field.
ISSN:2296-858X
2296-858X
DOI:10.3389/fmed.2024.1328474