Microwell bag culture for large-scale production of homogeneous islet-like clusters

Pluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 10 5 –10 6 islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to be overcome for its clinical...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2022-03, Vol.12 (1), p.5221-5221, Article 5221
Main Authors: Suenaga, Ryo, Konagaya, Shuhei, Yamaura, Junji, Ito, Ryo, Tanaka, Satoshi, Ishizaki, Yoichi, Toyoda, Taro
Format: Article
Language:English
Subjects:
631/61/2320
631/61/490
631/61/54
Cell culture
Degassing
Diabetes
Diabetes mellitus
Humanities and Social Sciences
Insulin
Islet cells
multidisciplinary
Permeability
Pharmaceutical industry
Pluripotency
R&D
Research & development
Science
Science (multidisciplinary)
Stem cells
Sterility
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 10 5 –10 6 islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to be overcome for its clinical use. In this study, we aimed to develop a novel bag culture method for the production of uniform cell clusters on a large scale (10 5 –10 6 clusters). We prepared small-scale culture bags (
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-09124-w