Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis

• Published in: Scientific reports 2021-04, Vol.11 (1), p.7411-7411, Article 7411
• Main Authors: Martínez-Matías, Nelson, Chorna, Nataliya, González-Crespo, Sahily, Villanueva, Lilliam, Montes-Rodríguez, Ingrid, Melendez-Aponte, Loyda M., Roche-Lima, Abiel, Carrasquillo-Carrión, Kelvin, Santiago-Cartagena, Ednalise, Rymond, Brian C., Babu, Mohan, Stagljar, Igor, Rodríguez-Medina, José R.
• Format: Article
• Language: English
• Subjects: 631/114; 631/1647; 631/326; 631/337; 631/45; 631/553; 631/80; Amino acids; Carboxylic acids; Cell walls; Chromatography, Liquid; Computational Biology - methods; Data Curation; Enzymes; Gene Expression Profiling - methods; Heat shock proteins; Heat tolerance; Homeostasis; Humanities and Social Sciences; Mechanotransduction, Cellular; Metabolism; Metabolites; Metabolomics - methods; Mitochondria; multidisciplinary; Oxidative stress; Protein Interaction Mapping; Protein Interaction Maps; Proteins; Proteogenomics - methods; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Science; Science (multidisciplinary); Tandem Mass Spectrometry; Trehalose; Yeast; Yeasts
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-86671-8

Functional analysis of the Mtl1 protein in Saccharomyces cerevisiae has revealed that this transmembrane sensor endows yeast cells with resistance to oxidative stress through a signaling mechanism called the cell wall integrity pathway (CWI). We observed upregulation of multiple heat shock proteins (HSPs), proteins associated with the formation of stress granules, and the phosphatase subunit of trehalose 6-phosphate synthase which suggests that mtl1Δ strains undergo intrinsic activation of a non-lethal heat stress response. Furthermore, quantitative global proteomic analysis conducted on TMT-labeled proteins combined with metabolome analysis revealed that mtl1Δ strains exhibit decreased levels of metabolites of carboxylic acid metabolism, decreased expression of anabolic enzymes and increased expression of catabolic enzymes involved in the metabolism of amino acids, with enhanced expression of mitochondrial respirasome proteins. These observations support the idea that Mtl1 protein controls the suppression of a non-lethal heat stress response under normal conditions while it plays an important role in metabolic regulatory mechanisms linked to TORC1 signaling that are required to maintain cellular homeostasis and optimal mitochondrial function.