2-Oxoglutarate contributes to the effect of foliar nitrogen on enhancing drought tolerance during flowering and grain yield of soybean

• Published in: Scientific reports 2023-05, Vol.13 (1), p.7274-7274, Article 7274
• Main Authors: Gai, Zhijia, Zhang, Maoming, Zhang, Pengfei, Zhang, Jingtao, Liu, Jingqi, Cai, Lijun, Yang, Xu, Zhang, Na, Yan, Zhengnan, Liu, Lei, Feng, Guozhong
• Format: Article
• Language: English
• Subjects: 631/449/1736; 631/449/2661; Antioxidants - metabolism; Crop yield; Drought; Drought Resistance; Droughts; Flowering; Glutamate-ammonia ligase; Glutamine; Glycine max - metabolism; Humanities and Social Sciences; Ketoglutaric acid; Ketoglutaric Acids - pharmacology; Malondialdehyde; multidisciplinary; Nitrogen; Nitrogen - pharmacology; Photosynthesis; Plant growth; Science; Science (multidisciplinary); Soybeans
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-34403-5

Drought severely affects the growth and yield of soybean plants especially during the flowering period. To investigate the effect of 2-oxoglutarate (2OG) in combination with foliar nitrogen (N) at flowering stage on drought resistance and seed yield of soybean under drought stress. This experiment was conducted in 2021 and 2022 on drought-resistant variety (Hefeng 50) and drought-sensitive variety (Hefeng 43) soybean plants treated with foliar N (DS + N) and 2-oxoglutarate (DS + 2OG) at flowering stage under drought stress. The results showed that drought stress at flowering stage significantly increased leaf malonaldehyde (MDA) content and reduced soybean yield per plant. However, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were significantly increased by foliar N treatment, and 2-oxoglutarate synergistically with foliar N treatment (DS + N + 2OG) was more beneficial to plant photosynthesis. 2-oxoglutarate significantly enhanced plant N content, glutamine synthetase (GS) and glutamate synthase (GOGAT) activity. Furthermore, 2-oxoglutarate increased the accumulation of proline and soluble sugars under drought stress. Under drought stress, soybean seed yield was increased by DS + N + 2OG treatment by 16.48–17.10% and 14.96–18.84% in 2021 and 2022, respectively. Thus, the combination of foliar N and 2-oxoglutarate better mitigated the adverse effects of drought stress and could better compensate for the yield loss of soybean under drought stress.