Location of aquaporins 3, 7 and 10 in frozen-thawed ejaculated and cauda epididymal spermatozoa from the Iberian ibex, mouflon, and chamois

Spermatozoa collected from the cauda epididymis of wild ruminants are more cryoresistant than ejaculated spermatozoa. Changes in the membrane location of aquaporins (AQPs) follow the osmotic changes that occur during freeze-thawing, and might influence the cryosurvival of spermatozoa depending on th...

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Bibliographic Details
Published in:Theriogenology Wild (Online) 2023, Vol.2, p.100025, Article 100025
Main Authors: Pequeño, Belén, Martínez-Madrid, Belén, Castaño, Cristina, Toledano-Díaz, Adolfo, Bóveda, Paula, Esteso, Milagros C., Gómez-Guillamón, Félix, Prieto, Paloma, Marcos-Beltrán, Jaime L., Alvarez-Rodriguez, Manuel, Rodriguez-Martinez, Heriberto, Santiago-Moreno, Julián
Format: Article
Language:English
Subjects:
acrosome
animal reproduction
Aquaporins
Capra
chamois
Cryopreservation
droplets
epididymis
freeze-thaw cycles
immunocytochemistry
mouflon
rabbits
Sperm, cryosurvival
Wild ruminants
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Summary:Spermatozoa collected from the cauda epididymis of wild ruminants are more cryoresistant than ejaculated spermatozoa. Changes in the membrane location of aquaporins (AQPs) follow the osmotic changes that occur during freeze-thawing, and might influence the cryosurvival of spermatozoa depending on their source. This work reports the location of AQP3, AQP7 and AQP10 in the cauda epididymal and post-ejaculation spermatozoa of three wild mountain ungulate species (Iberian ibex, mouflon, and chamois), as determined by Western blotting (WB) and immunocytochemistry (ICC) using commercial rabbit polyclonal primary antibodies. WB confirmed the presence of all three AQPs in the spermatozoa of all the studied species, while ICC showed AQP3 to be mainly located in the sperm acrosome, mid-piece, principal piece, and end piece, both in cauda epididymal and ejaculated cells. The percentage of ejaculated spermatozoa showing AQP3 in the principal piece was higher in the ibex than in the chamois (P 
ISSN:2773-093X
2773-093X
DOI:10.1016/j.therwi.2023.100025