Carbohydrate and amino acid metabolism and oxidative status in Holstein heifers precision-fed diets with different forage to concentrate ratios

• Published in: Animal (Cambridge, England) England), 2020-11, Vol.14 (11), p.2315-2325
• Main Authors: Zhang, J., Shi, H. T., Wang, Y. C., Li, S. L., Cao, Z. J., Yang, H. J., Wang, Y. J.
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - metabolism; Animal Feed; Animals; Antioxidants; Carbohydrate metabolism; Carbohydrates; Cattle - metabolism; Chromatography; Dairy cattle; Diet; Diet - veterinary; Digestion; Efficiency; Feed efficiency; Feeds; Female; Forage; Gas chromatography; heifer; high concentrate; Hydrogen peroxide; Hydroxyl radicals; Metabolism; Metabolites; Metabolomics; Nutrient deficiency; Nutrition; Oxidative metabolism; Oxidative Stress; Plasma; redox; Research Article; Rumen - metabolism; Superoxide dismutase
• ISSN: 1751-7311; 1751-732X
• DOI: 10.1017/S1751731120001287

Previous work led to the proposal that the precision feeding of a high-concentrate diet may represent a potential method with which to enhance feed efficiency (FE) when rearing dairy heifers. However, the physiological and metabolic mechanisms underlying this approach remain unclear. This study used metabolomics analysis to investigate the changes in plasma metabolites of heifers precision-fed diets containing a wide range of forage to concentrate ratios. Twenty-four half-sib Holstein heifers, with a similar body condition, were randomly assigned into four groups and precision fed with diets containing different proportions of concentrate (20%, 40%, 60% and 80% based on DM). After 28 days of feeding, blood samples were collected 6 h after morning feeding and gas chromatography time-of-flight/MS was used to analyze the plasma samples. Parameters of oxidative status were also determined in the plasma. The FE (after being corrected for gut fill) increased linearly (P < 0.01) with increasing level of dietary concentrate. Significant changes were identified for 38 different metabolites in the plasma of heifers fed different dietary forage to concentrate ratios. The main pathways showing alterations were clustered into those relating to carbohydrate and amino acid metabolism; all of which have been previously associated with FE changes in ruminants. Heifers fed with a high-concentrate diet had higher (P < 0.01) plasma total antioxidant capacity and superoxide dismutase but lower (P ≤ 0.02) hydroxyl radical and hydrogen peroxide than heifers fed with a low-concentrate diet, which might indicate a lower plasma oxidative status in the heifers fed a high-concentrate diet. Thus, heifers fed with a high-concentrate diet had higher FE and antioxidant capacity but a lower plasma oxidative status as well as changed carbohydrate and amino acid metabolism. Our findings provide a better understanding of how forage to concentrate ratios affect FE and metabolism in the precision-fed growing heifers.