Loading…

Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids

Pancreatic ductal adenocarcinoma (PDAC) is a cancer with a meager prognosis due to its chemotherapy resistance. A new treatment method may be magnetic fluid hyperthermia (MFH). Magnetoliposomes (ML), consisting of superparamagnetic iron oxide nanoparticles (SPION) stabilized with a phospholipid-bila...

Full description

Saved in:
Bibliographic Details
Published in:International journal of nanomedicine 2021, Vol.16, p.2965-2981
Main Authors: Palzer, Julian, Mues, Benedikt, Goerg, Richard, Aberle, Merel, Rensen, Sander S, Olde Damink, Steven W M, Vaes, Rianne D W, Cramer, Thorsten, Schmitz-Rode, Thomas, Neumann, Ulf P, Slabu, Ioana, Roeth, Anjali A
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c5239-4f4d7cfd1e3e585f1b03e3f43d22c602807ff00af565542a79494dc2d8b62b673
cites cdi_FETCH-LOGICAL-c5239-4f4d7cfd1e3e585f1b03e3f43d22c602807ff00af565542a79494dc2d8b62b673
container_end_page 2981
container_issue
container_start_page 2965
container_title International journal of nanomedicine
container_volume 16
creator Palzer, Julian
Mues, Benedikt
Goerg, Richard
Aberle, Merel
Rensen, Sander S
Olde Damink, Steven W M
Vaes, Rianne D W
Cramer, Thorsten
Schmitz-Rode, Thomas
Neumann, Ulf P
Slabu, Ioana
Roeth, Anjali A
description Pancreatic ductal adenocarcinoma (PDAC) is a cancer with a meager prognosis due to its chemotherapy resistance. A new treatment method may be magnetic fluid hyperthermia (MFH). Magnetoliposomes (ML), consisting of superparamagnetic iron oxide nanoparticles (SPION) stabilized with a phospholipid-bilayer, are exposed to an alternating magnetic field (AMF) to generate heat. To optimize this therapy, we investigated the effects of MFH on human PDAC cell lines and 3D organoid cultures. ML cytotoxicity was tested on Mia PaCa-2 and PANC-1 cells and on PDAC 3D organoid cultures, generated from resected tissue of patients. The MFH was achieved by AMF application with an amplitude of 40-47 kA/m and a frequency of 270 kHz. The MFH effect on the cell viability of the cell lines and the organoid cultures was investigated at two different time points. Clonogenic assays evaluated the impairment of colony formation. Altering ML set-ups addressed differences arising from intra- vs extracellular ML locations. Mia PaCa-2 and PANC-1 cells showed no cytotoxic effects at ML concentrations up to 300 µg(Fe)/mL and 225 µg(Fe)/mL, respectively. ML at a concentration of 225 µg(Fe)/mL were also non-toxic for PDAC organoid cultures. MFH treatment using exclusively extracellular ML presented the highest impact on cell viability. Clonogenic assays demonstrated remarkable impairment as long-term outcome in MFH-treated PDAC cell lines. Additionally, we successfully treated PDAC organoids with extracellular ML-derived MFH, resulting in notably reduced cell viabilities 2h and 24 h post treatment. Still, PDAC organoids seem to partly recover from MFH after 24 h as opposed to conventional 2D-cultures. Treatment with MFH strongly diminished pancreatic cancer cell viability in vitro, making it a promising treatment strategy. As organoids resemble the more advanced in vivo conditions better than conventional 2D cell lines, our organoid model holds great potential for further investigations.
doi_str_mv 10.2147/ijn.s288379
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_f860a1105eec46e8b39e92f746f39649</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A669417779</galeid><doaj_id>oai_doaj_org_article_f860a1105eec46e8b39e92f746f39649</doaj_id><sourcerecordid>A669417779</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5239-4f4d7cfd1e3e585f1b03e3f43d22c602807ff00af565542a79494dc2d8b62b673</originalsourceid><addsrcrecordid>eNptkk1vEzEQhlcIREvhxB2txBEl-NvrC1IVURpUCBLlbHntceqwawd7g9R_j0NKaaTKB49m3nn0zmia5jVGc4KZfB82cV5I11GpnjSnGMtuRhCmTx_EJ82LUjYIcdkJ9bw5oVRRzpQ4bX5-MesIU7DtxbALrr283UKebiCPwbSmtNcZzDRCnNrVdgoptj7l9puJdp-vXYsaQm4XMAylNdE9UlvltYkpuPKyeebNUODV3X_W_Lj4eL24nF2tPi0X51czywlVM-aZk9Y7DBR4xz3uEQXqGXWEWIFIh6T3CBnPBeeMGKmYYs4S1_WC9ELSs2Z54LpkNnqbw2jyrU4m6L-JlNfa5GpwAO07gQzGiANYJqDrqQJFvGTCUyWYqqwPB9Z214_gbN1ENsMR9LgSw41ep9-6uqw7phXw9g6Q068dlElv0i7HOr8mnHREMsTxf9XaVFch-lRhdgzF6nMhFMNSyr2Z-SOq-hyMwaYIPtT8UcO7Q4PNqZQM_t44Rnp_PHr5-av-fjieqn7zcNZ77b9roX8AG4e-mA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2528274051</pqid></control><display><type>article</type><title>Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids</title><source>Taylor &amp; Francis Open Access</source><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Palzer, Julian ; Mues, Benedikt ; Goerg, Richard ; Aberle, Merel ; Rensen, Sander S ; Olde Damink, Steven W M ; Vaes, Rianne D W ; Cramer, Thorsten ; Schmitz-Rode, Thomas ; Neumann, Ulf P ; Slabu, Ioana ; Roeth, Anjali A</creator><creatorcontrib>Palzer, Julian ; Mues, Benedikt ; Goerg, Richard ; Aberle, Merel ; Rensen, Sander S ; Olde Damink, Steven W M ; Vaes, Rianne D W ; Cramer, Thorsten ; Schmitz-Rode, Thomas ; Neumann, Ulf P ; Slabu, Ioana ; Roeth, Anjali A</creatorcontrib><description>Pancreatic ductal adenocarcinoma (PDAC) is a cancer with a meager prognosis due to its chemotherapy resistance. A new treatment method may be magnetic fluid hyperthermia (MFH). Magnetoliposomes (ML), consisting of superparamagnetic iron oxide nanoparticles (SPION) stabilized with a phospholipid-bilayer, are exposed to an alternating magnetic field (AMF) to generate heat. To optimize this therapy, we investigated the effects of MFH on human PDAC cell lines and 3D organoid cultures. ML cytotoxicity was tested on Mia PaCa-2 and PANC-1 cells and on PDAC 3D organoid cultures, generated from resected tissue of patients. The MFH was achieved by AMF application with an amplitude of 40-47 kA/m and a frequency of 270 kHz. The MFH effect on the cell viability of the cell lines and the organoid cultures was investigated at two different time points. Clonogenic assays evaluated the impairment of colony formation. Altering ML set-ups addressed differences arising from intra- vs extracellular ML locations. Mia PaCa-2 and PANC-1 cells showed no cytotoxic effects at ML concentrations up to 300 µg(Fe)/mL and 225 µg(Fe)/mL, respectively. ML at a concentration of 225 µg(Fe)/mL were also non-toxic for PDAC organoid cultures. MFH treatment using exclusively extracellular ML presented the highest impact on cell viability. Clonogenic assays demonstrated remarkable impairment as long-term outcome in MFH-treated PDAC cell lines. Additionally, we successfully treated PDAC organoids with extracellular ML-derived MFH, resulting in notably reduced cell viabilities 2h and 24 h post treatment. Still, PDAC organoids seem to partly recover from MFH after 24 h as opposed to conventional 2D-cultures. Treatment with MFH strongly diminished pancreatic cancer cell viability in vitro, making it a promising treatment strategy. As organoids resemble the more advanced in vivo conditions better than conventional 2D cell lines, our organoid model holds great potential for further investigations.</description><identifier>ISSN: 1178-2013</identifier><identifier>ISSN: 1176-9114</identifier><identifier>EISSN: 1178-2013</identifier><identifier>DOI: 10.2147/ijn.s288379</identifier><identifier>PMID: 33935496</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>Adenocarcinoma - pathology ; Adenocarcinoma - therapy ; Biocompatibility ; Cancer ; Cancer therapies ; Carcinoma, Pancreatic Ductal - metabolism ; Carcinoma, Pancreatic Ductal - pathology ; Cell culture ; Cell Death ; Cell Line, Tumor ; Cell Survival ; Chemotherapy ; Clone Cells ; Drug therapy ; Efficiency ; Ferric oxide ; Fever ; Humans ; Hyperthermia ; Hyperthermia, Induced ; Magnetic fields ; magnetic fluid hyperthermia ; magnetic nanoparticles ; Magnetic Phenomena ; Medical prognosis ; Nanoparticles ; organoids ; Organoids - pathology ; Original Research ; Pancreatic cancer ; Pancreatic Neoplasms ; Pancreatic Neoplasms - pathology ; Pancreatic Neoplasms - therapy ; pdac ; Prognosis ; spion ; Tumors</subject><ispartof>International journal of nanomedicine, 2021, Vol.16, p.2965-2981</ispartof><rights>2021 Palzer et al.</rights><rights>COPYRIGHT 2021 Dove Medical Press Limited</rights><rights>2021. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Palzer et al. 2021 Palzer et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5239-4f4d7cfd1e3e585f1b03e3f43d22c602807ff00af565542a79494dc2d8b62b673</citedby><cites>FETCH-LOGICAL-c5239-4f4d7cfd1e3e585f1b03e3f43d22c602807ff00af565542a79494dc2d8b62b673</cites><orcidid>0000-0003-1990-1784 ; 0000-0002-1181-2165 ; 0000-0002-1670-7487 ; 0000-0002-5457-7628 ; 0000-0003-4513-8698 ; 0000-0002-5202-9345</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2528274051/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2528274051?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,25753,27923,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33935496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palzer, Julian</creatorcontrib><creatorcontrib>Mues, Benedikt</creatorcontrib><creatorcontrib>Goerg, Richard</creatorcontrib><creatorcontrib>Aberle, Merel</creatorcontrib><creatorcontrib>Rensen, Sander S</creatorcontrib><creatorcontrib>Olde Damink, Steven W M</creatorcontrib><creatorcontrib>Vaes, Rianne D W</creatorcontrib><creatorcontrib>Cramer, Thorsten</creatorcontrib><creatorcontrib>Schmitz-Rode, Thomas</creatorcontrib><creatorcontrib>Neumann, Ulf P</creatorcontrib><creatorcontrib>Slabu, Ioana</creatorcontrib><creatorcontrib>Roeth, Anjali A</creatorcontrib><title>Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids</title><title>International journal of nanomedicine</title><addtitle>Int J Nanomedicine</addtitle><description>Pancreatic ductal adenocarcinoma (PDAC) is a cancer with a meager prognosis due to its chemotherapy resistance. A new treatment method may be magnetic fluid hyperthermia (MFH). Magnetoliposomes (ML), consisting of superparamagnetic iron oxide nanoparticles (SPION) stabilized with a phospholipid-bilayer, are exposed to an alternating magnetic field (AMF) to generate heat. To optimize this therapy, we investigated the effects of MFH on human PDAC cell lines and 3D organoid cultures. ML cytotoxicity was tested on Mia PaCa-2 and PANC-1 cells and on PDAC 3D organoid cultures, generated from resected tissue of patients. The MFH was achieved by AMF application with an amplitude of 40-47 kA/m and a frequency of 270 kHz. The MFH effect on the cell viability of the cell lines and the organoid cultures was investigated at two different time points. Clonogenic assays evaluated the impairment of colony formation. Altering ML set-ups addressed differences arising from intra- vs extracellular ML locations. Mia PaCa-2 and PANC-1 cells showed no cytotoxic effects at ML concentrations up to 300 µg(Fe)/mL and 225 µg(Fe)/mL, respectively. ML at a concentration of 225 µg(Fe)/mL were also non-toxic for PDAC organoid cultures. MFH treatment using exclusively extracellular ML presented the highest impact on cell viability. Clonogenic assays demonstrated remarkable impairment as long-term outcome in MFH-treated PDAC cell lines. Additionally, we successfully treated PDAC organoids with extracellular ML-derived MFH, resulting in notably reduced cell viabilities 2h and 24 h post treatment. Still, PDAC organoids seem to partly recover from MFH after 24 h as opposed to conventional 2D-cultures. Treatment with MFH strongly diminished pancreatic cancer cell viability in vitro, making it a promising treatment strategy. As organoids resemble the more advanced in vivo conditions better than conventional 2D cell lines, our organoid model holds great potential for further investigations.</description><subject>Adenocarcinoma - pathology</subject><subject>Adenocarcinoma - therapy</subject><subject>Biocompatibility</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Carcinoma, Pancreatic Ductal - metabolism</subject><subject>Carcinoma, Pancreatic Ductal - pathology</subject><subject>Cell culture</subject><subject>Cell Death</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Chemotherapy</subject><subject>Clone Cells</subject><subject>Drug therapy</subject><subject>Efficiency</subject><subject>Ferric oxide</subject><subject>Fever</subject><subject>Humans</subject><subject>Hyperthermia</subject><subject>Hyperthermia, Induced</subject><subject>Magnetic fields</subject><subject>magnetic fluid hyperthermia</subject><subject>magnetic nanoparticles</subject><subject>Magnetic Phenomena</subject><subject>Medical prognosis</subject><subject>Nanoparticles</subject><subject>organoids</subject><subject>Organoids - pathology</subject><subject>Original Research</subject><subject>Pancreatic cancer</subject><subject>Pancreatic Neoplasms</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Pancreatic Neoplasms - therapy</subject><subject>pdac</subject><subject>Prognosis</subject><subject>spion</subject><subject>Tumors</subject><issn>1178-2013</issn><issn>1176-9114</issn><issn>1178-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNptkk1vEzEQhlcIREvhxB2txBEl-NvrC1IVURpUCBLlbHntceqwawd7g9R_j0NKaaTKB49m3nn0zmia5jVGc4KZfB82cV5I11GpnjSnGMtuRhCmTx_EJ82LUjYIcdkJ9bw5oVRRzpQ4bX5-MesIU7DtxbALrr283UKebiCPwbSmtNcZzDRCnNrVdgoptj7l9puJdp-vXYsaQm4XMAylNdE9UlvltYkpuPKyeebNUODV3X_W_Lj4eL24nF2tPi0X51czywlVM-aZk9Y7DBR4xz3uEQXqGXWEWIFIh6T3CBnPBeeMGKmYYs4S1_WC9ELSs2Z54LpkNnqbw2jyrU4m6L-JlNfa5GpwAO07gQzGiANYJqDrqQJFvGTCUyWYqqwPB9Z214_gbN1ENsMR9LgSw41ep9-6uqw7phXw9g6Q068dlElv0i7HOr8mnHREMsTxf9XaVFch-lRhdgzF6nMhFMNSyr2Z-SOq-hyMwaYIPtT8UcO7Q4PNqZQM_t44Rnp_PHr5-av-fjieqn7zcNZ77b9roX8AG4e-mA</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Palzer, Julian</creator><creator>Mues, Benedikt</creator><creator>Goerg, Richard</creator><creator>Aberle, Merel</creator><creator>Rensen, Sander S</creator><creator>Olde Damink, Steven W M</creator><creator>Vaes, Rianne D W</creator><creator>Cramer, Thorsten</creator><creator>Schmitz-Rode, Thomas</creator><creator>Neumann, Ulf P</creator><creator>Slabu, Ioana</creator><creator>Roeth, Anjali A</creator><general>Dove Medical Press Limited</general><general>Taylor &amp; Francis Ltd</general><general>Dove</general><general>Dove Medical Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1990-1784</orcidid><orcidid>https://orcid.org/0000-0002-1181-2165</orcidid><orcidid>https://orcid.org/0000-0002-1670-7487</orcidid><orcidid>https://orcid.org/0000-0002-5457-7628</orcidid><orcidid>https://orcid.org/0000-0003-4513-8698</orcidid><orcidid>https://orcid.org/0000-0002-5202-9345</orcidid></search><sort><creationdate>2021</creationdate><title>Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids</title><author>Palzer, Julian ; Mues, Benedikt ; Goerg, Richard ; Aberle, Merel ; Rensen, Sander S ; Olde Damink, Steven W M ; Vaes, Rianne D W ; Cramer, Thorsten ; Schmitz-Rode, Thomas ; Neumann, Ulf P ; Slabu, Ioana ; Roeth, Anjali A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5239-4f4d7cfd1e3e585f1b03e3f43d22c602807ff00af565542a79494dc2d8b62b673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adenocarcinoma - pathology</topic><topic>Adenocarcinoma - therapy</topic><topic>Biocompatibility</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Carcinoma, Pancreatic Ductal - metabolism</topic><topic>Carcinoma, Pancreatic Ductal - pathology</topic><topic>Cell culture</topic><topic>Cell Death</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>Chemotherapy</topic><topic>Clone Cells</topic><topic>Drug therapy</topic><topic>Efficiency</topic><topic>Ferric oxide</topic><topic>Fever</topic><topic>Humans</topic><topic>Hyperthermia</topic><topic>Hyperthermia, Induced</topic><topic>Magnetic fields</topic><topic>magnetic fluid hyperthermia</topic><topic>magnetic nanoparticles</topic><topic>Magnetic Phenomena</topic><topic>Medical prognosis</topic><topic>Nanoparticles</topic><topic>organoids</topic><topic>Organoids - pathology</topic><topic>Original Research</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Pancreatic Neoplasms - therapy</topic><topic>pdac</topic><topic>Prognosis</topic><topic>spion</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palzer, Julian</creatorcontrib><creatorcontrib>Mues, Benedikt</creatorcontrib><creatorcontrib>Goerg, Richard</creatorcontrib><creatorcontrib>Aberle, Merel</creatorcontrib><creatorcontrib>Rensen, Sander S</creatorcontrib><creatorcontrib>Olde Damink, Steven W M</creatorcontrib><creatorcontrib>Vaes, Rianne D W</creatorcontrib><creatorcontrib>Cramer, Thorsten</creatorcontrib><creatorcontrib>Schmitz-Rode, Thomas</creatorcontrib><creatorcontrib>Neumann, Ulf P</creatorcontrib><creatorcontrib>Slabu, Ioana</creatorcontrib><creatorcontrib>Roeth, Anjali A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palzer, Julian</au><au>Mues, Benedikt</au><au>Goerg, Richard</au><au>Aberle, Merel</au><au>Rensen, Sander S</au><au>Olde Damink, Steven W M</au><au>Vaes, Rianne D W</au><au>Cramer, Thorsten</au><au>Schmitz-Rode, Thomas</au><au>Neumann, Ulf P</au><au>Slabu, Ioana</au><au>Roeth, Anjali A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids</atitle><jtitle>International journal of nanomedicine</jtitle><addtitle>Int J Nanomedicine</addtitle><date>2021</date><risdate>2021</risdate><volume>16</volume><spage>2965</spage><epage>2981</epage><pages>2965-2981</pages><issn>1178-2013</issn><issn>1176-9114</issn><eissn>1178-2013</eissn><abstract>Pancreatic ductal adenocarcinoma (PDAC) is a cancer with a meager prognosis due to its chemotherapy resistance. A new treatment method may be magnetic fluid hyperthermia (MFH). Magnetoliposomes (ML), consisting of superparamagnetic iron oxide nanoparticles (SPION) stabilized with a phospholipid-bilayer, are exposed to an alternating magnetic field (AMF) to generate heat. To optimize this therapy, we investigated the effects of MFH on human PDAC cell lines and 3D organoid cultures. ML cytotoxicity was tested on Mia PaCa-2 and PANC-1 cells and on PDAC 3D organoid cultures, generated from resected tissue of patients. The MFH was achieved by AMF application with an amplitude of 40-47 kA/m and a frequency of 270 kHz. The MFH effect on the cell viability of the cell lines and the organoid cultures was investigated at two different time points. Clonogenic assays evaluated the impairment of colony formation. Altering ML set-ups addressed differences arising from intra- vs extracellular ML locations. Mia PaCa-2 and PANC-1 cells showed no cytotoxic effects at ML concentrations up to 300 µg(Fe)/mL and 225 µg(Fe)/mL, respectively. ML at a concentration of 225 µg(Fe)/mL were also non-toxic for PDAC organoid cultures. MFH treatment using exclusively extracellular ML presented the highest impact on cell viability. Clonogenic assays demonstrated remarkable impairment as long-term outcome in MFH-treated PDAC cell lines. Additionally, we successfully treated PDAC organoids with extracellular ML-derived MFH, resulting in notably reduced cell viabilities 2h and 24 h post treatment. Still, PDAC organoids seem to partly recover from MFH after 24 h as opposed to conventional 2D-cultures. Treatment with MFH strongly diminished pancreatic cancer cell viability in vitro, making it a promising treatment strategy. As organoids resemble the more advanced in vivo conditions better than conventional 2D cell lines, our organoid model holds great potential for further investigations.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>33935496</pmid><doi>10.2147/ijn.s288379</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0003-1990-1784</orcidid><orcidid>https://orcid.org/0000-0002-1181-2165</orcidid><orcidid>https://orcid.org/0000-0002-1670-7487</orcidid><orcidid>https://orcid.org/0000-0002-5457-7628</orcidid><orcidid>https://orcid.org/0000-0003-4513-8698</orcidid><orcidid>https://orcid.org/0000-0002-5202-9345</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1178-2013
ispartof International journal of nanomedicine, 2021, Vol.16, p.2965-2981
issn 1178-2013
1176-9114
1178-2013
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_f860a1105eec46e8b39e92f746f39649
source Taylor & Francis Open Access; Publicly Available Content Database; PubMed Central
subjects Adenocarcinoma - pathology
Adenocarcinoma - therapy
Biocompatibility
Cancer
Cancer therapies
Carcinoma, Pancreatic Ductal - metabolism
Carcinoma, Pancreatic Ductal - pathology
Cell culture
Cell Death
Cell Line, Tumor
Cell Survival
Chemotherapy
Clone Cells
Drug therapy
Efficiency
Ferric oxide
Fever
Humans
Hyperthermia
Hyperthermia, Induced
Magnetic fields
magnetic fluid hyperthermia
magnetic nanoparticles
Magnetic Phenomena
Medical prognosis
Nanoparticles
organoids
Organoids - pathology
Original Research
Pancreatic cancer
Pancreatic Neoplasms
Pancreatic Neoplasms - pathology
Pancreatic Neoplasms - therapy
pdac
Prognosis
spion
Tumors
title Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T00%3A15%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Magnetic%20Fluid%20Hyperthermia%20as%20Treatment%20Option%20for%20Pancreatic%20Cancer%20Cells%20and%20Pancreatic%20Cancer%20Organoids&rft.jtitle=International%20journal%20of%20nanomedicine&rft.au=Palzer,%20Julian&rft.date=2021&rft.volume=16&rft.spage=2965&rft.epage=2981&rft.pages=2965-2981&rft.issn=1178-2013&rft.eissn=1178-2013&rft_id=info:doi/10.2147/ijn.s288379&rft_dat=%3Cgale_doaj_%3EA669417779%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5239-4f4d7cfd1e3e585f1b03e3f43d22c602807ff00af565542a79494dc2d8b62b673%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2528274051&rft_id=info:pmid/33935496&rft_galeid=A669417779&rfr_iscdi=true