A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis , ESV200, a transient biofilm producer

causes gallibacteriosis in birds. These bacteria produce biofilms and secrete several fimbrial appendages as tools to cause disease in animals. strains contain up to three types of fimbriae. is the strategy currently used to determine variations in the gene content of , although today only the compl...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in microbiology 2023-01, Vol.14, p.1084766-1084766
Main Authors: Sanchez-Alonso, Patricia, Cobos-Justo, Elena, Avalos-Rangel, Miguel Angel, López-Reyes, Lucía, Paniagua-Contreras, Gloria Luz, Vaca-Paniagua, Felipe, Anastacio-Marcelino, Estela, López-Ochoa, Ana Jaqueline, Pérez Marquez, Victor M, Negrete-Abascal, Erasmo, Vázquez-Cruz, Candelario
Format: Article
Language:English
Subjects:
avian diseases
biofilm
fimbriae
Gallibacterium anatis
genome
Microbiology
pathogenesis
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:causes gallibacteriosis in birds. These bacteria produce biofilms and secrete several fimbrial appendages as tools to cause disease in animals. strains contain up to three types of fimbriae. is the strategy currently used to determine variations in the gene content of , although today only the completely circularized genome of UMN179 is available. The appearance of growth of various strains of in liquid culture medium was studied. Biofilm production and how the amount of biofilm was affected by DNase, Proteinase K, and Pronase E enzymes were analyzed. Fimbrial gene expression was performed by protein analysis and qRT-PCR. In an avian model, the pathogenesis generated by the strains ESV200 and 12656-12 was investigated. Using bioinformatic tools, the complete genome of G. anatis ESV200 was comparatively studied to search for virulence factors that would help explain the pathogenic behavior of this strain. ESV200 strain differs from the 12656-12 strain because it produces a biofilm at 20%. ESV200 strain express fimbrial genes and produces biofilm but with a different structure than that observed for strain 12656-12. ESV200 and 12656-12 strains are pathogenic for chickens, although the latter is the most virulent. Here, we show that the complete genome of the ESV200 strain is similar to that of the UNM179 strain. However, these strains have evolved with many structural rearrangements; the most striking chromosomal arrangement is a Maverick-like element present in the ESV200 strain.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2023.1084766