Gravidity influences distinct transcriptional profiles of maternal and fetal placental macrophages at term

• Published in: Frontiers in immunology 2024-06, Vol.15, p.1384361
• Main Authors: Ozarslan, Nida, Robinson, Joshua F, Buarpung, Sirirak, Kim, M Yvonne, Ansbro, Megan R, Akram, Jason, Montoya, Dennis J, Kamya, Moses R, Kakuru, Abel, Dorsey, Grant, Rosenthal, Philip J, Cheng, Genhong, Feeney, Margaret E, Fisher, Susan J, Gaw, Stephanie L
• Format: Article
• Language: English
• Subjects: Adult; Female; Fetus - immunology; Gene Expression Profiling; gravidity; Hofbauer cell; Humans; Immunology; Macrophages - immunology; Macrophages - metabolism; monocyte; Monocytes - immunology; Monocytes - metabolism; myeloid cells; placenta; Placenta - immunology; Placenta - metabolism; Pregnancy; Transcriptome
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2024.1384361

Maternal intervillous monocytes (MIMs) and fetal Hofbauer cells (HBCs) are myeloid-derived immune cells at the maternal-fetal interface. Maternal reproductive history is associated with differential risk of pregnancy complications. The molecular phenotypes and roles of these distinct monocyte/macrophage populations and the influence of gravidity on these phenotypes has not been systematically investigated. Here, we used RNA sequencing to study the transcriptional profiles of MIMs and HBCs in normal term pregnancies. Our analyses revealed distinct transcriptomes of MIMs and HBCs. Genes involved in differentiation and cell organization pathways were more highly expressed in MIMs vs. HBCs. In contrast, HBCs had higher expression of genes involved in inflammatory responses and cell surface receptor signaling. Maternal gravidity influenced monocyte programming, as expression of pro-inflammatory molecules was significantly higher in MIMs from multigravidae compared to primigravidae. In HBCs, multigravidae displayed enrichment of gene pathways involved in cell-cell signaling and differentiation. Our results demonstrated that MIMs and HBCs have highly divergent transcriptional signatures, reflecting their distinct origins, locations, functions, and roles in inflammatory responses. Furthermore, maternal gravidity influences the gene signatures of MIMs and HBCs, potentially modulating the interplay between tolerance and trained immunity. The phenomenon of reproductive immune memory may play a novel role in the differential susceptibility of primigravidae to pregnancy complications.