Apoptosis Related Human Wharton's Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells-Complete Transcriptomic Assays

Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) exhibit multilineage differentiation potential, adhere to plastic, and express a specific set of surface markers-CD105, CD73, CD90. Although there are relatively well-established differentiation protocols for WJ-MSCs, the exact molecular...

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Published in:International journal of molecular sciences 2023-06, Vol.24 (12), p.10023
Main Authors: Stefańska, Katarzyna, Nemcova, Lucie, Blatkiewicz, Małgorzata, Pieńkowski, Wojciech, Ruciński, Marcin, Zabel, Maciej, Mozdziak, Paul, Podhorska-Okołów, Marzenna, Dzięgiel, Piotr, Kempisty, Bartosz
Format: Article
Language:English
Subjects:
Adipocytes
Analysis
Apoptosis
CD105 antigen
CD14 antigen
CD73 antigen
Cell culture
Cell differentiation
Chondrocytes
Chondrogenesis
Culture
Differentiation
Extracellular matrix
Flow cytometry
FOXO1 protein
Genes
Mesenchymal stem cells
Molecular modelling
MSC
Osteoblastogenesis
Pharmaceutical industry
Regenerative medicine
RNA sequencing
RNAseq
Stem cell research
Stem cells
Surface markers
Transcriptomics
Umbilical cord
Wharton’s jelly
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Summary:Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) exhibit multilineage differentiation potential, adhere to plastic, and express a specific set of surface markers-CD105, CD73, CD90. Although there are relatively well-established differentiation protocols for WJ-MSCs, the exact molecular mechanisms involved in their in vitro long-term culture and differentiation remain to be elucidated. In this study, the cells were isolated from Wharton's jelly of umbilical cords obtained from healthy full-term deliveries, cultivated in vitro, and differentiated towards osteogenic, chondrogenic, adipogenic and neurogenic lineages. RNA samples were isolated after the differentiation regimen and analyzed using an RNA sequencing (RNAseq) assay, which led to the identification of differentially expressed genes belonging to apoptosis-related ontological groups. and were upregulated in all differentiated groups as compared to controls, while was downregulated in all groups. In addition, several possible novel marker genes associated with the differentiation of WJ-MSCs were identified (e.g., , , , , , ). The results of this study provide an insight into the molecular mechanisms involved in the long-term culture in vitro and four-lineage differentiation of WJ-MSCs, which is crucial to utilize WJ-MSCs in regenerative medicine.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241210023