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Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents...
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Published in: | Frontiers in physiology 2021-02, Vol.12, p.642237 |
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creator | Martins, Michele Ramos, Luis Felipe Costa Murillo, Jimmy Rodriguez Torres, André de Carvalho, Stephanie Serafim Domont, Gilberto Barbosa de Oliveira, Danielle Maria Perpétua Mesquita, Rafael Dias Nogueira, Fábio César Sousa Maciel-de-Freitas, Rafael Junqueira, Magno |
description | Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector,
, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont
has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which
orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection;
infection; co-infection with
ZIKV in the
heads and salivary glands. We show that
regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of
in mosquitoes was determined by MS and corroborates the idea that
helps to block ZIKV infections in
The present study offers a rich resource of data that may help to elucidate mechanisms by which
orchestrate resistance to ZIKV infection in
, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and
directly in complex tissues. |
doi_str_mv | 10.3389/fphys.2021.642237 |
format | article |
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, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont
has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which
orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection;
infection; co-infection with
ZIKV in the
heads and salivary glands. We show that
regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of
in mosquitoes was determined by MS and corroborates the idea that
helps to block ZIKV infections in
The present study offers a rich resource of data that may help to elucidate mechanisms by which
orchestrate resistance to ZIKV infection in
, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and
directly in complex tissues.</description><identifier>ISSN: 1664-042X</identifier><identifier>EISSN: 1664-042X</identifier><identifier>DOI: 10.3389/fphys.2021.642237</identifier><identifier>PMID: 33716790</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>Aedes aegypti ; immune response ; Physiology ; proteome ; quantitative ; Wolbachia ; Zika virus</subject><ispartof>Frontiers in physiology, 2021-02, Vol.12, p.642237</ispartof><rights>Copyright © 2021 Martins, Ramos, Murillo, Torres, de Carvalho, Domont, de Oliveira, Mesquita, Nogueira, Maciel-de-Freitas and Junqueira.</rights><rights>Copyright © 2021 Martins, Ramos, Murillo, Torres, de Carvalho, Domont, de Oliveira, Mesquita, Nogueira, Maciel-de-Freitas and Junqueira. 2021 Martins, Ramos, Murillo, Torres, de Carvalho, Domont, de Oliveira, Mesquita, Nogueira, Maciel-de-Freitas and Junqueira</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c271t-8317df2c60d186d9f652f0260c9fc35833f17849e332bc307e3ce680b081c5483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947915/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947915/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33716790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martins, Michele</creatorcontrib><creatorcontrib>Ramos, Luis Felipe Costa</creatorcontrib><creatorcontrib>Murillo, Jimmy Rodriguez</creatorcontrib><creatorcontrib>Torres, André</creatorcontrib><creatorcontrib>de Carvalho, Stephanie Serafim</creatorcontrib><creatorcontrib>Domont, Gilberto Barbosa</creatorcontrib><creatorcontrib>de Oliveira, Danielle Maria Perpétua</creatorcontrib><creatorcontrib>Mesquita, Rafael Dias</creatorcontrib><creatorcontrib>Nogueira, Fábio César Sousa</creatorcontrib><creatorcontrib>Maciel-de-Freitas, Rafael</creatorcontrib><creatorcontrib>Junqueira, Magno</creatorcontrib><title>Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon</title><title>Frontiers in physiology</title><addtitle>Front Physiol</addtitle><description>Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector,
, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont
has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which
orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection;
infection; co-infection with
ZIKV in the
heads and salivary glands. We show that
regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of
in mosquitoes was determined by MS and corroborates the idea that
helps to block ZIKV infections in
The present study offers a rich resource of data that may help to elucidate mechanisms by which
orchestrate resistance to ZIKV infection in
, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and
directly in complex tissues.</description><subject>Aedes aegypti</subject><subject>immune response</subject><subject>Physiology</subject><subject>proteome</subject><subject>quantitative</subject><subject>Wolbachia</subject><subject>Zika virus</subject><issn>1664-042X</issn><issn>1664-042X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkt9u2yAUxq1p01q1fYDdTLxAMv7YYN9MiqKti1RpndS1024QgUNM54AFTiY_0N5zON6qlhvg8H2_A-IrincELxmrmw-2b8e0pJiSJS8pZeJVcU44Lxe4pD9eP1ufFVcpPeI8SkwxJm-LM8YE4aLB58Wfddj3EVrwyR0BfTsoP7hBDdPmNoYBwh7QyqtuTC6hYNEKDCSkYDf2g0MbA1lvXS6d1M7nM2_QrRra32pMaOOPoTuCQc6jh9BtlW6dQr3r3WScxT_dL4XuXTxM8gGihQhe5_75Vrm9D_6yeGNVl-Dq33xRfP_86W79ZXHz9XqzXt0sNBVkWNSMCGOp5tiQmpvG8opaTDnWjdWsqhmzRNRlA4zRrWZYANPAa7zFNdFVWbOLYjNzTVCPso9ur-Iog3LyVAhxJ1UcnO5A2rrBwCwGLmxpNFG0MkyxMrOF1gZn1seZ1R-2ezA6vzeq7gX05Yl3rdyFoxRNKRpSZQCZATqGlCLYJy_BcoqAPEVAThGQcwSy5_3zpk-O_x_O_gJTxrMB</recordid><startdate>20210225</startdate><enddate>20210225</enddate><creator>Martins, Michele</creator><creator>Ramos, Luis Felipe Costa</creator><creator>Murillo, Jimmy Rodriguez</creator><creator>Torres, André</creator><creator>de Carvalho, Stephanie Serafim</creator><creator>Domont, Gilberto Barbosa</creator><creator>de Oliveira, Danielle Maria Perpétua</creator><creator>Mesquita, Rafael Dias</creator><creator>Nogueira, Fábio César Sousa</creator><creator>Maciel-de-Freitas, Rafael</creator><creator>Junqueira, Magno</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20210225</creationdate><title>Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon</title><author>Martins, Michele ; Ramos, Luis Felipe Costa ; Murillo, Jimmy Rodriguez ; Torres, André ; de Carvalho, Stephanie Serafim ; Domont, Gilberto Barbosa ; de Oliveira, Danielle Maria Perpétua ; Mesquita, Rafael Dias ; Nogueira, Fábio César Sousa ; Maciel-de-Freitas, Rafael ; Junqueira, Magno</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c271t-8317df2c60d186d9f652f0260c9fc35833f17849e332bc307e3ce680b081c5483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aedes aegypti</topic><topic>immune response</topic><topic>Physiology</topic><topic>proteome</topic><topic>quantitative</topic><topic>Wolbachia</topic><topic>Zika virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martins, Michele</creatorcontrib><creatorcontrib>Ramos, Luis Felipe Costa</creatorcontrib><creatorcontrib>Murillo, Jimmy Rodriguez</creatorcontrib><creatorcontrib>Torres, André</creatorcontrib><creatorcontrib>de Carvalho, Stephanie Serafim</creatorcontrib><creatorcontrib>Domont, Gilberto Barbosa</creatorcontrib><creatorcontrib>de Oliveira, Danielle Maria Perpétua</creatorcontrib><creatorcontrib>Mesquita, Rafael Dias</creatorcontrib><creatorcontrib>Nogueira, Fábio César Sousa</creatorcontrib><creatorcontrib>Maciel-de-Freitas, Rafael</creatorcontrib><creatorcontrib>Junqueira, Magno</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martins, Michele</au><au>Ramos, Luis Felipe Costa</au><au>Murillo, Jimmy Rodriguez</au><au>Torres, André</au><au>de Carvalho, Stephanie Serafim</au><au>Domont, Gilberto Barbosa</au><au>de Oliveira, Danielle Maria Perpétua</au><au>Mesquita, Rafael Dias</au><au>Nogueira, Fábio César Sousa</au><au>Maciel-de-Freitas, Rafael</au><au>Junqueira, Magno</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon</atitle><jtitle>Frontiers in physiology</jtitle><addtitle>Front Physiol</addtitle><date>2021-02-25</date><risdate>2021</risdate><volume>12</volume><spage>642237</spage><pages>642237-</pages><issn>1664-042X</issn><eissn>1664-042X</eissn><abstract>Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector,
, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont
has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which
orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection;
infection; co-infection with
ZIKV in the
heads and salivary glands. We show that
regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of
in mosquitoes was determined by MS and corroborates the idea that
helps to block ZIKV infections in
The present study offers a rich resource of data that may help to elucidate mechanisms by which
orchestrate resistance to ZIKV infection in
, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and
directly in complex tissues.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>33716790</pmid><doi>10.3389/fphys.2021.642237</doi><oa>free_for_read</oa></addata></record> |
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subjects | Aedes aegypti immune response Physiology proteome quantitative Wolbachia Zika virus |
title | Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon |
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