The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity

Synaptic vesicle (SV) release probability ( ) is a key presynaptic determinant of synaptic strength established by cell-intrinsic properties and further refined by plasticity. To characterize mechanisms that generate heterogeneity between distinct neuronal populations, we examined glutamatergic toni...

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Bibliographic Details
Published in:eLife 2021-10, Vol.10
Main Authors: Sauvola, Chad W, Akbergenova, Yulia, Cunningham, Karen L, Aponte-Santiago, Nicole A, Littleton, J Troy
Format: Article
Language:English
Subjects:
Animals
Brain
Controlled release
CRISPR
Drosophila
Drosophila melanogaster - physiology
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Fruit-flies
Genomes
Glutamatergic transmission
Homeostasis - genetics
Homeostatic plasticity
Injuries
Insects
Male
Motor neurons
Mutation
N-Ethylmaleimide
Neuronal Plasticity
Neuroscience
neurotransmitter release
Potentiation
Proteins
SNAP receptors
snare complex
SNARE Proteins - genetics
SNARE Proteins - metabolism
synapse
Synapses
Synaptic depression
Synaptic plasticity
Synaptic strength
synaptic vesicle
Tomosyn
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Summary:Synaptic vesicle (SV) release probability ( ) is a key presynaptic determinant of synaptic strength established by cell-intrinsic properties and further refined by plasticity. To characterize mechanisms that generate heterogeneity between distinct neuronal populations, we examined glutamatergic tonic (Ib) and phasic (Is) motoneurons in with stereotyped differences in and synaptic plasticity. We found the decoy soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) Tomosyn is differentially expressed between these motoneuron subclasses and contributes to intrinsic differences in their synaptic output. Tomosyn expression enables tonic release in Ib motoneurons by reducing SNARE complex formation and suppressing to generate decreased levels of SV fusion and enhanced resistance to synaptic fatigue. In contrast, phasic release dominates when Tomosyn expression is low, enabling high intrinsic at Is terminals at the expense of sustained release and robust presynaptic potentiation. In addition, loss of Tomosyn disrupts the ability of tonic synapses to undergo presynaptic homeostatic potentiation.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.72841