Chemosensory Proteins Are Associated with Thiamethoxam and Spirotetramat Tolerance in Aphis gossypii Glover

• Published in: International journal of molecular sciences 2022-02, Vol.23 (4), p.2356
• Main Authors: Xu, Hongfei, Yan, Kunpeng, Ding, Yaping, Lv, Yuntong, Li, Jianyi, Yang, Fengting, Chen, Xuewei, Gao, Xiwu, Pan, Yiou, Shang, Qingli
• Format: Article
• Language: English
• Subjects: Animals; Animals, Genetically Modified; Aphidoidea; Aphids - drug effects; Aphids - metabolism; Aphids - physiology; Aphis gossypii; Arthropods; Aza Compounds - metabolism; Bioassays; Chemoreception; chemosensory protein; Cotton; Cypermethrin; Drosophila melanogaster - genetics; Fruit flies; Gene expression; Insect Proteins - metabolism; Insecticide Resistance; Insecticides; Insecticides - metabolism; Insects; Membrane Transport Proteins - metabolism; Metabolism; Mortality; Peptides; Pest resistance; Pesticides; Proteins; RNA-mediated interference; Sensitivity; Spiro Compounds - metabolism; spirotetramat; Thiamethoxam; Thiamethoxam - metabolism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23042356

Chemosensory proteins (CSPs) are a class of transporters in arthropods. Deeper research on CSPs showed that CSPs may be involved in some physiological processes beyond chemoreception, such as insect resistance to pesticides. We identified two upregulated in two resistant strains of Glover. To understand their role in the resistance of aphids to pesticides, we performed the functional verification of and in vivo and in vitro. Results showed that the sensitivity of the thiamethoxam-resistant strain to thiamethoxam increased significantly with the silencing of and by RNAi (RNA interference), and the sensitivity of the spirotetramat-resistant strain to spirotetramat increased significantly with the silencing of . Transgenic expressing exhibited stronger resistance to thiamethoxam, spirotetramat, and alpha-cypermethrin than the control did. In the bioassay of transgenic , showed different tolerance mechanisms for different pesticides, and the overexpressed may play a role in processes other than resistance to pesticides. In brief, the present results prove that are related to the resistance of cotton aphids to insecticides.