Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles

• Published in: Frontiers in microbiology 2022-07, Vol.13, p.934708-934708
• Main Authors: Prescott, Rebecca D., Zamkovaya, Tatyana, Donachie, Stuart P., Northup, Diana E., Medley, Joseph J., Monsalve, Natalia, Saw, Jimmy H., Decho, Alan W., Chain, Patrick S. G., Boston, Penelope J.
• Format: Article
• Language: English
• Subjects: cave microbiology; fumaroles; lava caves; microbial consortia; Microbiology; networks; taxonomic distinctness
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2022.934708

Lava caves, tubes, and fumaroles in Hawai‘i present a range of volcanic, oligotrophic environments from different lava flows and host unexpectedly high levels of bacterial diversity. These features provide an opportunity to study the ecological drivers that structure bacterial community diversity and assemblies in volcanic ecosystems and compare the older, more stable environments of lava tubes, to the more variable and extreme conditions of younger, geothermally active caves and fumaroles. Using 16S rRNA amplicon-based sequencing methods, we investigated the phylogenetic distinctness and diversity and identified microbial interactions and consortia through co-occurrence networks in 70 samples from lava tubes, geothermal lava caves, and fumaroles on the island of Hawai‘i. Our data illustrate that lava caves and geothermal sites harbor unique microbial communities, with very little overlap between caves or sites. We also found that older lava tubes (500–800 yrs old) hosted greater phylogenetic diversity (Faith's PD) than sites that were either geothermally active or younger (