Cerium Oxide Nanoparticles and Their Efficient Antibacterial Application In Vitro against Gram-Positive and Gram-Negative Pathogens

• Published in: Nanomaterials (Basel, Switzerland) Switzerland), 2020-08, Vol.10 (8), p.1614
• Main Authors: Pop, Oana L., Mesaros, Amalia, Vodnar, Dan C., Suharoschi, Ramona, Tăbăran, Flaviu, Magerușan, Lidia, Tódor, István Sz, Diaconeasa, Zoriţa, Balint, Adriana, Ciontea, Lelia, Socaciu, Carmen
• Format: Article
• Language: English
• Subjects: Acids; Antibacterial activity; Antimicrobial agents; antioxidant activity; Antioxidants; Aqueous solutions; Bacillus cereus; Bacteria; Cerium; cerium oxide nanoparticles; Cerium oxides; Chemical precipitation; Chemical synthesis; Differential thermal analysis; E coli; Energy gap; Escherichia coli; Foodborne pathogens; Fourier transforms; Growth media; Lasers; Listeria; Listeria monocytogenes; Metal oxides; Methods; Microorganisms; Morphology; Nanoparticles; Nitrates; Pathogens; Physical characteristics; Raman spectroscopy; Salmonella; Salmonella Typhimurium; Spectrum analysis; Staphylococcus aureus; Thermal analysis; X-ray diffraction
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano10081614

In this study, the antibacterial activity of cerium oxide nanoparticles on two Gram-negative and three Gram-positive foodborne pathogens was investigated. CeO2 nanoparticles (CeO2 nps) were synthesized by a Wet Chemical Synthesis route, using the precipitation method and the Simultaneous Addition of reactants (WCS–SimAdd). The as-obtained precursor powders were investigated by thermal analysis (TG–DTA), to study their decomposition process and to understand the CeO2 nps formation. The composition, structure, and morphology of the thermally treated sample were investigated by FTIR, Raman spectroscopy, X-ray diffraction, TEM, and DLS. The cubic structure and average particle size ranging between 5 and 15 nm were evidenced. Optical absorption measurements (UV–Vis) reveal that the band gap of CeO2 is 2.61 eV, which is smaller than the band gap of bulk ceria. The antioxidant effect of CeO2 nps was determined, and the antibacterial test was carried out both in liquid and on solid growth media against five pathogenic microorganisms, namely Escherichia coli, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Cerium oxide nanoparticles showed growth inhibition toward all five pathogens tested with notable results. This paper highlights the perspectives for the synthesis of CeO2 nps with controlled structural and morphological characteristics and enhanced antibacterial properties, using a versatile and low-cost chemical solution method.