Humoral response and evolution of Echinococcus infection in experimentally infected sheep

There is little information on the humoral response of sheep experimentally infected with Echinococcus granulosus. Thus, the objective of this study was to evaluate this response and measure its evolution. Doses of 10, 100, 1000 and 10000 E. granulosus eggs were prepared and inoculated via intrarumi...

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Published in:Revista brasileira de parasitologia veterinaria 2014-04, Vol.23 (2), p.237-240
Main Authors: Lamberti, Roberto, Cavagion, Laura, Gatti, Antonio, Calvo, Claudio, Gino, Lilia, Puches, Veronica Véspoli, Alvarez, Angela Rosa, Alvarez, Emiliano, Cachau, Mariela García, Morete, Mariela, Larrieu, Edmundo
Format: Article
Language:English
Subjects:
Animal Experimentation
Animals
Echinococcosis
Echinococcosis - immunology
Echinococcosis - veterinary
Echinococcus granulosus
humoral response
Immunity, Humoral
PARASITOLOGY
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Sheep
Sheep Diseases - immunology
Sheep Diseases - parasitology
VETERINARY SCIENCES
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Summary:There is little information on the humoral response of sheep experimentally infected with Echinococcus granulosus. Thus, the objective of this study was to evaluate this response and measure its evolution. Doses of 10, 100, 1000 and 10000 E. granulosus eggs were prepared and inoculated via intraruminal puncture. Blood samples were obtained before inoculation and every 48 h after inoculation, until they became seropositive. Thereafter, they were taken monthly for the first year and then every three months until 1700 days of observation had been completed. An ELISA test, with total hydatid fluid antigen, was used for immunodiagnosis. The average optical density of the 12 inoculated sheep was found to be above the mean cutoff value 10 days after inoculation, went on increasing until 180 days after inoculation and remained above the cutoff level until the end of the observation period. This confirms that the antibody response of sheep to E. granulosus infection occurs before production of hydatid fluid and that activation, mobilization and establishment of oncospheres in the tissues generates a persistent response from the host's immune system.
ISSN:1984-2961
1984-2961
DOI:10.1590/S1984-29612014030