Understanding the responses of tillering to 2,4-D isooctyl ester in Setaria viridis L

• Published in: BMC genomics 2024-07, Vol.25 (1), p.682-12, Article 682
• Main Authors: Xiong, Wangdan, Jia, Xinfeng, Wang, Qixin, Zhong, Nina, Gao, Hanchi, Zhang, Lingxin, Sun, Juan
• Format: Article
• Language: English
• Subjects: 2,4-D; 2,4-D isooctyl ester; 2,4-Dichlorophenoxyacetic Acid - pharmacology; Abscisic acid; Agrochemicals; Alfalfa; Analysis; Auxins; Cellular signal transduction; Cytokinins; Defoliants; Distribution; Esters; Farm buildings; Gene expression; Gene Expression Regulation, Plant - drug effects; Genes; Genetic aspects; Genetic engineering; Gibberellins; Gibberellins - metabolism; Gibberellins - pharmacology; Grasses; Herbicides; Herbicides - pharmacology; Hormones; Indoleacetic Acids - metabolism; Indoleacetic Acids - pharmacology; Isooctyl ester; Leaves; Metabolism; Photosynthesis - drug effects; Phytohormones; Plant growth; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; Plant hormones; Plant Leaves - drug effects; Plant Leaves - metabolism; Setaria Plant - drug effects; Setaria Plant - genetics; Setaria Plant - growth & development; Setaria Plant - metabolism; Setaria viridis; Signal transduction; Signal Transduction - drug effects; Target recognition; Tillering; Tillers; Transcriptome - drug effects; Transcriptomes; Weeds
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10579-6

Green foxtail [Setaria viridis (L.)] is one of the most abundant and troublesome annual grass weeds in alfalfa fields in Northeast China. Synthetic auxin herbicide is widely used in agriculture, while how auxin herbicide affects tillering on perennial grass weeds is still unclear. A greenhouse experiment was conducted to examine the effects of auxin herbicide 2,4-D on green foxtail growth, especially on tillers. In the study, 2,4-D isooctyl ester was used. There was an inhibition of plant height and fresh weight on green foxtail after application. The photosynthetic rate of the leaves was dramatically reduced and there was an accumulation of malondialdehyde (MDA) content. Moreover, applying 2,4-D isooctyl ester significantly reduced the tillering buds at rates between 2100 and 8400 ga. i. /ha. Transcriptome results showed that applying 2,4-D isooctyl ester on leaves affected the phytohormone signal transduction pathways in plant tillers. Among them, there were significant effects on auxin, cytokinin, abscisic acid (ABA), gibberellin (GA), and brassinosteroid signaling. Indeed, external ABA and GA on leaves also limited tillering in green foxtail. These data will be helpful to further understand the responses of green foxtail to 2, 4-D isooctyl ester, which may provide a unique perspective for the development and identification of new target compounds that are effective against this weed species.