SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy

• Published in: Virology journal 2021-07, Vol.18 (1), p.1-149, Article 149
• Main Authors: Mondeja, Brian, Valdes, Odalys, Resik, Sonia, Vizcaino, Ananayla, Acosta, Emilio, Montalván, Adelmo, Paez, Amira, Mune, Mayra, Rodríguez, Roberto, Valdés, Juan, Gonzalez, Guelsys, Sanchez, Daisy, Falcón, Viviana, González, Yorexis, Kourí, Vivian, Díaz, Angelina, Guzmán, María
• Format: Article
• Language: English
• Subjects: AFM; Algorithms; Anosmia; Antigens; Atomic force microscopy; Cell membranes; Cell surface; Comparative analysis; Confocal microscopy; Coronavirus; Coronaviruses; COVID-19; Cytology; Epithelial cells; Health aspects; Image processing; Laboratories; Microscope and microscopy; Microscopy; Morphology; Observations; Olfaction disorders; Physical characteristics; Polymerase chain reaction; SARS-CoV-2; Scanning electron microscopy; Segmentation; SEM; Severe acute respiratory syndrome coronavirus 2; Short Report; Virions; Virulence (Microbiology); Virus research; Viruses
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-021-01620-1

The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. Nasopharyngeal swabs were collected from 12 Cuban individuals, six asymptomatic and RT-PCR negative (negative control) and six others from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by scanning electron microscopy (SEM), confocal microscopy (CM) and, atomic force microscopy. Improvement and segmentation of coronavirus images were performed by a novel mathematical image enhancement algorithm. The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions budding through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by SEM. Viral antigen was identified in the apical cells zone by CM. The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to nasopharyngeal samples awaits future use.