Double vision: 2D and 3D mosquito trajectories can be as valuable for behaviour analysis via machine learning

• Published in: Parasites & vectors 2024-07, Vol.17 (1), p.282-15, Article 282
• Main Authors: Qureshi, Yasser Mehmood, Voloshin, Vitaly, Towers, Catherine Elizabeth, Covington, James Anthony, Towers, David Peter
• Format: Article
• Language: English
• Subjects: Animals; Behavior, Animal; Behaviour; Control; Culicidae - classification; Culicidae - physiology; Disease transmission; Entomologists; Equipment and supplies; Female; Identification and classification; Image processing; Imaging systems; Imaging, Three-Dimensional - methods; Machine Learning; Machine vision; Male; Methodology; Methods; Mosquito tracking; Mosquito Vectors - classification; Mosquito Vectors - physiology; Mosquitoes; Practice; Prevention; Trajectories
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-024-06356-9

Mosquitoes are carriers of tropical diseases, thus demanding a comprehensive understanding of their behaviour to devise effective disease control strategies. In this article we show that machine learning can provide a performance assessment of 2D and 3D machine vision techniques and thereby guide entomologists towards appropriate experimental approaches for behaviour assessment. Behaviours are best characterised via tracking-giving a full time series of information. However, tracking systems vary in complexity. Single-camera imaging yields two-component position data which generally are a function of all three orthogonal components due to perspective; however, a telecentric imaging setup gives constant magnification with respect to depth and thereby measures two orthogonal position components. Multi-camera or holographic techniques quantify all three components. In this study a 3D mosquito mating swarm dataset was used to generate equivalent 2D data via telecentric imaging and a single camera at various imaging distances. The performance of the tracking systems was assessed through an established machine learning classifier that differentiates male and non-male mosquito tracks. SHAPs analysis has been used to explore the trajectory feature values for each model. The results reveal that both telecentric and single-camera models, when placed at large distances from the flying mosquitoes, can produce equivalent accuracy from a classifier as well as preserve characteristic features without resorting to more complex 3D tracking techniques. Caution should be exercised when employing a single camera at short distances as classifier balanced accuracy is reduced compared to that from 3D or telecentric imaging; the trajectory features also deviate compared to those from the other datasets. It is postulated that measurement of two orthogonal motion components is necessary to optimise the accuracy of machine learning classifiers based on trajectory data. The study increases the evidence base for using machine learning to determine behaviours from insect trajectory data.