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Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18
Paracoccidioides brasiliensis , a thermal dimorphic fungal pathogen, produces a melanin-like pigment in vitro and in vivo. We investigated the involvement of carbohydrates and monoclonal antibody to CD18, on phagocytosis inhibition, involving macrophage receptors and the resistance of melanized fung...
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| Published in: | Memórias do Instituto Oswaldo Cruz 2009-07, Vol.104 (4), p.644-648 |
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| Main Authors: | , , , , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-b590t-9a17aeeef965ffb0ab7d646adf4d7466082d89fe6e75fb29bd4f66bd033ff22e3 |
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| container_issue | 4 |
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| container_title | Memórias do Instituto Oswaldo Cruz |
| container_volume | 104 |
| creator | da Silva, Marcelo Barbosa Thomaz, Luciana Marques, Alexandre Ferreira Svidzinski, Artur E Nosanchuk, Josh D Casadevall, Arturo Travassos, Luiz R Taborda, Carlos P |
| description | Paracoccidioides brasiliensis , a thermal dimorphic fungal pathogen,
produces a melanin-like pigment in vitro and in vivo. We investigated
the involvement of carbohydrates and monoclonal antibody to CD18, on
phagocytosis inhibition, involving macrophage receptors and the
resistance of melanized fungal cells to chemically generated nitric
oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our
results demonstrate that melanized yeast cells were more resistant than
nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite
and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually
abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were
added together in this system. Intratracheal infection of BALB/c mice,
with melanized yeast cells, resulted in higher lung colony forming
units, when compared to nonmelanized yeast cells. Therefore, melanin is
a virulence factor of P. brasiliensis. |
| doi_str_mv | 10.1590/s0074-02762009000400019 |
| format | article |
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produces a melanin-like pigment in vitro and in vivo. We investigated
the involvement of carbohydrates and monoclonal antibody to CD18, on
phagocytosis inhibition, involving macrophage receptors and the
resistance of melanized fungal cells to chemically generated nitric
oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our
results demonstrate that melanized yeast cells were more resistant than
nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite
and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually
abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were
added together in this system. Intratracheal infection of BALB/c mice,
with melanized yeast cells, resulted in higher lung colony forming
units, when compared to nonmelanized yeast cells. Therefore, melanin is
a virulence factor of P. brasiliensis.</description><identifier>ISSN: 1678-8060</identifier><identifier>ISSN: 0074-0276</identifier><identifier>EISSN: 1678-8060</identifier><identifier>EISSN: 0074-0276</identifier><identifier>DOI: 10.1590/s0074-02762009000400019</identifier><identifier>PMID: 19722091</identifier><language>eng</language><publisher>Brazil: Fundação Oswaldo Cruz, Fiocruz</publisher><subject>Animals ; anti-CD18 antibody ; Antibodies, Monoclonal - pharmacology ; Antifungal Agents - pharmacology ; Antimicrobial agents ; antimicrobial oxidants ; Carbohydrates - pharmacology ; CD18 Antigens - drug effects ; Macrophages - microbiology ; melanin ; melanin - P. brasiliensis - antimicrobial oxidants - anti-CD18 antibody ; Melanins - biosynthesis ; Mice ; Mice, Inbred BALB C ; Oxidants - pharmacology ; P. brasiliensis ; Paracoccidioides - drug effects ; Paracoccidioides - metabolism ; Paracoccidioides - pathogenicity ; Paracoccidioides brasiliensis ; PARASITOLOGY ; Phagocytosis ; TROPICAL MEDICINE ; Virulence Factors - physiology</subject><ispartof>Memórias do Instituto Oswaldo Cruz, 2009-07, Vol.104 (4), p.644-648</ispartof><rights>Copyright 2009 - Instituto Oswaldo Cruz - Fiocruz</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b590t-9a17aeeef965ffb0ab7d646adf4d7466082d89fe6e75fb29bd4f66bd033ff22e3</citedby><cites>FETCH-LOGICAL-b590t-9a17aeeef965ffb0ab7d646adf4d7466082d89fe6e75fb29bd4f66bd033ff22e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,24150,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19722091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva, Marcelo Barbosa</creatorcontrib><creatorcontrib>Thomaz, Luciana</creatorcontrib><creatorcontrib>Marques, Alexandre Ferreira</creatorcontrib><creatorcontrib>Svidzinski, Artur E</creatorcontrib><creatorcontrib>Nosanchuk, Josh D</creatorcontrib><creatorcontrib>Casadevall, Arturo</creatorcontrib><creatorcontrib>Travassos, Luiz R</creatorcontrib><creatorcontrib>Taborda, Carlos P</creatorcontrib><title>Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18</title><title>Memórias do Instituto Oswaldo Cruz</title><addtitle>Mem Inst Oswaldo Cruz</addtitle><description>Paracoccidioides brasiliensis , a thermal dimorphic fungal pathogen,
produces a melanin-like pigment in vitro and in vivo. We investigated
the involvement of carbohydrates and monoclonal antibody to CD18, on
phagocytosis inhibition, involving macrophage receptors and the
resistance of melanized fungal cells to chemically generated nitric
oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our
results demonstrate that melanized yeast cells were more resistant than
nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite
and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually
abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were
added together in this system. Intratracheal infection of BALB/c mice,
with melanized yeast cells, resulted in higher lung colony forming
units, when compared to nonmelanized yeast cells. Therefore, melanin is
a virulence factor of P. brasiliensis.</description><subject>Animals</subject><subject>anti-CD18 antibody</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antimicrobial agents</subject><subject>antimicrobial oxidants</subject><subject>Carbohydrates - pharmacology</subject><subject>CD18 Antigens - drug effects</subject><subject>Macrophages - microbiology</subject><subject>melanin</subject><subject>melanin - P. brasiliensis - antimicrobial oxidants - anti-CD18 antibody</subject><subject>Melanins - biosynthesis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Oxidants - pharmacology</subject><subject>P. brasiliensis</subject><subject>Paracoccidioides - drug effects</subject><subject>Paracoccidioides - metabolism</subject><subject>Paracoccidioides - pathogenicity</subject><subject>Paracoccidioides brasiliensis</subject><subject>PARASITOLOGY</subject><subject>Phagocytosis</subject><subject>TROPICAL MEDICINE</subject><subject>Virulence Factors - physiology</subject><issn>1678-8060</issn><issn>0074-0276</issn><issn>1678-8060</issn><issn>0074-0276</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9UtuO0zAQjRCIXRZ-AfIET13GjmMnj6jLZaWVQFyeLV_G7VRpXOxUovwVf4hDyy4S0kpj2To-54zHM1X1gsEla3t4nQGUWABXkgP0ACDKYv2D6pxJ1S06kPDwn_NZ9STnDRR-I8Xj6oz1inPo2Xn16zNmypMZHdYx1FsczEg_0dcHNHmqHQ5Dni8-mWRcdI48RfKYa5tMpoFwLPJ6irUZJ9qSS9GSGer4g3xBcoF9TeOaLE0Ux9lptzar6A5TnIX7TOOqdibZuD74ZCY8SrZxjG6IY7GajW30hznJ8op1T6tHwQwZn532i-rbu7dflx8WNx_fXy_f3Cxs-aBp0RumDCKGXrYhWDBWeSmk8UF4JaSEjvuuDyhRtcHy3noRpLQemiYEzrG5qK6Pvj6ajd4l2pp00NGQ_gPEtNImTeQG1KELomtZI7hnArjs2060fZBNA8o5NntdHr2yIxyi3sR9KrVl_WXuo_6vj0Xw6ijYpfh9j3nSW8pzM8yIcZ-1agSUUFCYL-9lcsahZa0sRHUklh7lnDDc1sRAz0N1z2Oen1Ls7Rb9ne40RXflWYoDjXjLcImM_gtGV6LQhWh-A3x42fg</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>da Silva, Marcelo Barbosa</creator><creator>Thomaz, Luciana</creator><creator>Marques, Alexandre Ferreira</creator><creator>Svidzinski, Artur E</creator><creator>Nosanchuk, Josh D</creator><creator>Casadevall, Arturo</creator><creator>Travassos, Luiz R</creator><creator>Taborda, Carlos P</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><general>Instituto Oswaldo Cruz, Ministério da Saúde</general><general>Fundação Oswaldo Cruz (FIOCRUZ)</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20090701</creationdate><title>Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18</title><author>da Silva, Marcelo Barbosa ; 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produces a melanin-like pigment in vitro and in vivo. We investigated
the involvement of carbohydrates and monoclonal antibody to CD18, on
phagocytosis inhibition, involving macrophage receptors and the
resistance of melanized fungal cells to chemically generated nitric
oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our
results demonstrate that melanized yeast cells were more resistant than
nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite
and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually
abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were
added together in this system. Intratracheal infection of BALB/c mice,
with melanized yeast cells, resulted in higher lung colony forming
units, when compared to nonmelanized yeast cells. Therefore, melanin is
a virulence factor of P. brasiliensis.</abstract><cop>Brazil</cop><pub>Fundação Oswaldo Cruz, Fiocruz</pub><pmid>19722091</pmid><doi>10.1590/s0074-02762009000400019</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1678-8060 |
| ispartof | Memórias do Instituto Oswaldo Cruz, 2009-07, Vol.104 (4), p.644-648 |
| issn | 1678-8060 0074-0276 1678-8060 0074-0276 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_f8f4851342d14026958459f63307cc1e |
| source | SciELO Brazil |
| subjects | Animals anti-CD18 antibody Antibodies, Monoclonal - pharmacology Antifungal Agents - pharmacology Antimicrobial agents antimicrobial oxidants Carbohydrates - pharmacology CD18 Antigens - drug effects Macrophages - microbiology melanin melanin - P. brasiliensis - antimicrobial oxidants - anti-CD18 antibody Melanins - biosynthesis Mice Mice, Inbred BALB C Oxidants - pharmacology P. brasiliensis Paracoccidioides - drug effects Paracoccidioides - metabolism Paracoccidioides - pathogenicity Paracoccidioides brasiliensis PARASITOLOGY Phagocytosis TROPICAL MEDICINE Virulence Factors - physiology |
| title | Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18 |
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