Boronic Acid-based approach for separation and immobilization of glycoproteins and its application in sensing

Glycoproteins influence a broad spectrum of biological processes including cell-cell interaction, host-pathogen interaction, or protection of proteins against proteolytic degradation. The analysis of their glyco-structures and concentration levels are increasingly important in diagnosis and proteomi...

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Bibliographic Details
Published in:International journal of molecular sciences 2013-10, Vol.14 (10), p.20890-20912
Main Authors: Wang, Xiaojin, Xia, Ning, Liu, Lin
Format: Article
Language:English
Subjects:
boronic acid
Boronic Acids - chemistry
glycoproteins
Glycoproteins - chemistry
Humans
Immobilization - methods
Review
sensing
separation
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Summary:Glycoproteins influence a broad spectrum of biological processes including cell-cell interaction, host-pathogen interaction, or protection of proteins against proteolytic degradation. The analysis of their glyco-structures and concentration levels are increasingly important in diagnosis and proteomics. Boronic acids can covalently react with cis-diols in the oligosaccharide chains of glycoproteins to form five- or six-membered cyclic esters. Based on this interaction, boronic acid-based ligands and materials have attracted much attention in both chemistry and biology as the recognition motif for enrichment and chemo/biosensing of glycoproteins in recent years. In this work, we reviewed the progress in the separation, immobilization and detection of glycoproteins with boronic acid-functionalized materials and addressed its application in sensing.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms141020890