The anterior insular cortex unilaterally controls feeding in response to aversive visceral stimuli in mice

• Published in: Nature communications 2020-01, Vol.11 (1), p.640-14, Article 640
• Main Authors: Wu, Yu, Chen, Changwan, Chen, Ming, Qian, Kai, Lv, Xinyou, Wang, Haiting, Jiang, Lifei, Yu, Lina, Zhuo, Min, Qiu, Shuang
• Format: Article
• Language: English
• Subjects: 13/1; 13/51; 14/19; 38/32; 42/34; 631/378; 631/378/1488; 631/378/3920; 64/60; 9/30; 9/74; Animal behavior; Animals; Anorexia; Aversive Agents - toxicity; Body weight loss; Ca2+/calmodulin-dependent protein kinase II; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; Cerebral Cortex - drug effects; Cerebral Cortex - physiology; Circuits; Cortex (insular); Feeding behavior; Feeding Behavior - drug effects; Female; Food; Food consumption; Food intake; Humanities and Social Sciences; Hypothalamic Area, Lateral - metabolism; Hypothalamus; Hypothalamus (lateral); Hypothalamus - cytology; Hypothalamus - drug effects; Hypothalamus - metabolism; Inhibition (psychology); Luteinizing hormone; Male; Mice; Mice, Inbred C57BL; multidisciplinary; Neural Pathways - drug effects; Neurons; Neurons - drug effects; Neurons - metabolism; Science; Science (multidisciplinary); Stimuli; Toxins; Viruses; Weight loss
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-14281-5

Reduced food intake is common to many pathological conditions, such as infection and toxin exposure. However, cortical circuits that mediate feeding responses to these threats are less investigated. The anterior insular cortex (aIC) is a core region that integrates interoceptive states and emotional awareness and consequently guides behavioral responses. Here, we demonstrate that the right-side aIC CamKII + (aIC CamKII ) neurons in mice are activated by aversive visceral signals. Hyperactivation of the right-side aIC CamKII neurons attenuates food consumption, while inhibition of these neurons increases feeding and reverses aversive stimuli-induced anorexia and weight loss. Similar manipulation at the left-side aIC does not cause significant behavioral changes. Furthermore, virus tracing reveals that aIC CamKII neurons project directly to the vGluT2 + neurons in the lateral hypothalamus (LH), and the right-side aIC CamKII -to-LH pathway mediates feeding suppression. Our studies uncover a circuit from the cortex to the hypothalamus that senses aversive visceral signals and controls feeding behavior. Food intake can be attenuated by visceral aversive stimuli in pathological conditions. Here the authors identify a unilateral neural circuit from the CamKII-positive neurons in the anterior insular cortex to the vGluT2-positive neurons in the lateral hypothalamus that controls feeding responses to visceral aversive stimuli.