Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution

White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus ( Mr NV), the causative agent of white tail...

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Bibliographic Details
Published in:Scientific reports 2017-05, Vol.7 (1), p.2083-8, Article 2083
Main Authors: Ho, Kok Lian, Kueh, Chare Li, Beh, Poay Ling, Tan, Wen Siang, Bhella, David
Format: Article
Language:English
Subjects:
101/28
631/326/596/2148
631/535/1258/1259
Animals
Aquaculture
Capsid - ultrastructure
Capsid protein
Developing countries
Electron microscopy
Farms
Hatcheries
Humanities and Social Sciences
LDCs
Macrobrachium rosenbergii
multidisciplinary
New genera
Nodaviridae - classification
Nodaviridae - ultrastructure
Palaemonidae - virology
Pest outbreaks
Science
Science (multidisciplinary)
Shellfish
Virus-like particles
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Summary:White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus ( Mr NV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent Mr NV disease however. Here we show the structure of Mr NV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that Mr NV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. Mr NV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that Mr NV may belong to a new genus of the Nodaviridae . Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-02292-0