Inducible clindamycin-resistant and biofilm formation in the Staphylococcus aureus isolated from healthcare worker's anterior nasal carriage

• Published in: BMC research notes 2024-09, Vol.17 (1), p.252-7, Article 252
• Main Authors: Firouzjaei, Mahdi Dadashi, Halaji, Mehrdad, Yaghoubi, Sajad, Hendizadeh, Peyman, Salehi, Maryam, Mohammadi, Mohsen, Pournajaf, Abazar
• Format: Article
• Language: English
• Subjects: Adult; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Antimicrobial agents; Biofilm; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Carrier State - microbiology; Ciprofloxacin; Clindamycin; Clindamycin - pharmacology; Cross infection; DNA methylation; Drug resistance; Drug resistance in microorganisms; Drug Resistance, Bacterial - genetics; Drug Resistance, Multiple, Bacterial - genetics; Erythromycin; Erythromycin - pharmacology; Female; Gene frequency; Genes; Health aspects; Health care; Health Personnel; Humans; Iran; Lincosamides; Macrolide–lincosamide–streptogramin B resistance; Male; Medical personnel; Medical research; Medical students; Medicine, Experimental; Methicillin; Methicillin-Resistant Staphylococcus aureus - drug effects; Methicillin-Resistant Staphylococcus aureus - genetics; Methicillin-Resistant Staphylococcus aureus - isolation & purification; Methicillin-Resistant Staphylococcus aureus - physiology; Microbial mats; Microbial Sensitivity Tests; MRSA; Nasal carriage; Nasal mucosa; Nosocomial infections; Research Note; Risk factors; S. Aureus; Staphylococcal Infections - epidemiology; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Staphylococcus aureus - physiology; Staphylococcus aureus infections; Staphylococcus infections; Statistical analysis; Streptogramin B; Streptogramins; Teaching hospitals
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-024-06926-1

The purpose of this study is a new update on the resistance profile, Macrolide-Lincosamide-Streptogramin B resistance mechanisms and biofilm formation in the Staphylococcus aureus isolated from health care workers (HCWs) nasal carriage at a children's teaching hospital in Babol (Northern Iran). A total of 143 non-repetitive nasal swab samples were collected from volunteers, where 53.8% (n; 77/143) were HCWs, 33.6% (n; 48/143) medical students, and 12.6% (n; 18/143) resident students. The prevalence of nasal carriers of S. aureus was 22.4% (n; 32/143), among them, 40.6% (n; 13/32) were identified as methicillin-resistant Staphylococcus aureus (MRSA( carriers. Antimicrobial susceptibility testing showed that erythromycin (68.8%, n; 22/32) and ciprofloxacin (15.6%, n; 5/32) had the highest and lowest resistance rate, respectively. The frequency of resistance genes in the strains was as follows; ermC (n; 17/32, 53.1%), ermA (n; 11/32, 34.4%), ermB (n; 6/32, 18.7%), ereA (n; 3/32, 9.4%). Moreover, 50.0% (n; 16/32), 28.1% (n; 9/32) and 21.8% (n; 7/32) of isolates were strongly, weakly and moderately biofilm producer, respectively. Macrolides-lincosamides-streptogramins B (MLSB) antibiotic resistance among S. aureus isolates from HCWs nasal carriage have found significant prevalence rates throughout the globe. It is crucial to remember that the development of biofilms and MLS B antibiotic resistance are both dynamic processes.