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Identification of Leishmania (Leishmania) chagasi isolated from healthy skin of symptomatic and asymptomatic dogs seropositive for leishmaniasis in the municipality of Rio de Janeiro, Brazil
Euthanasia of seropositive dogs has been one of the principal measures adopted by the Program for the Control of Leishmaniasis in Brazil for many years. However, its efficacy is currently being questioned. We obtained intact skin samples from 20 Leishmania-reactive dogs from the municipality of Rio...
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Published in: | The Brazilian journal of infectious diseases 2004-12, Vol.8 (6), p.440-444 |
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creator | Madeira, Maria de Fátima Schubach, Armando de Oliveira Schubach, Tânia Maria Pacheco Leal, Cristianni Antunes Marzochi, Mauro Célio de Almeida |
description | Euthanasia of seropositive dogs has been one of the principal measures adopted by the Program for the Control of Leishmaniasis in Brazil for many years. However, its efficacy is currently being questioned. We obtained intact skin samples from 20 Leishmania-reactive dogs from the municipality of Rio de Janeiro that had been referred for euthanasia. The promastigote forms of Leishmania were isolated in culture from 18 of these animals. Fourteen of these isolates were identified as Leishmania (Leishmania) chagasi by isoenzyme electrophoresis; seven of these were from asymptomatic dogs and seven were from symptomatic animals with visceral leishmaniasis. In conclusion, cutaneous parasitism is found in the intact skin of dogs naturally infected with L. (L.) chagasi, irrespective of the presence or absence of clinical signs suggestive of visceral leishmaniasis. |
doi_str_mv | 10.1590/S1413-86702004000600008 |
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However, its efficacy is currently being questioned. We obtained intact skin samples from 20 Leishmania-reactive dogs from the municipality of Rio de Janeiro that had been referred for euthanasia. The promastigote forms of Leishmania were isolated in culture from 18 of these animals. Fourteen of these isolates were identified as Leishmania (Leishmania) chagasi by isoenzyme electrophoresis; seven of these were from asymptomatic dogs and seven were from symptomatic animals with visceral leishmaniasis. In conclusion, cutaneous parasitism is found in the intact skin of dogs naturally infected with L. 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(L.) chagasi, irrespective of the presence or absence of clinical signs suggestive of visceral leishmaniasis.</description><subject>Animals</subject><subject>Brazil</subject><subject>clinical signs</subject><subject>dog</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>Electrophoresis - methods</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>INFECTIOUS DISEASES</subject><subject>intact skin</subject><subject>isoenzymes</subject><subject>Isoenzymes - analysis</subject><subject>Leishmania (Leishmania) chagasi</subject><subject>Leishmania infantum - isolation & purification</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Leishmaniasis, Visceral - veterinary</subject><issn>1413-8670</issn><issn>1678-4391</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9ksmOEzEQhvsAYoaBVwCfEEhkKNu9uI8wYgmKhMRytqq9JBW6243dQQoPx7PhSaIMEhIHy1a5_u8vu6oonnK45lULr77wksuFqhsQACUA1HmBuldcni8uiocpbQFElRMeFBe8UgqErC6L30vrxpk8GZwpjCx4tnKUNgOOhOz53fkFMxtcYyJGKfQ4O8t8DAPbOOznzZ6l73RQp_0wzWHINMNwtAz_DtiwTiy5GKaQaKafjvkQWX82SZRYxswbx4bdSIYm7Gne33I_U2DWsY84OorhJXsT8Rf1j4r7HvvkHp_2q-Lbu7dfbz4sVp_eL29erxZGKlALU4N3thMSpKklVKIsBbeu9lY0JVrRgeFGYcl9Z6yqrJdWNXXbSCU4B9nJq2J55NqAWz1FGjDudUDSh0CIa40xv7B32rfGYMuVhbYsq6bC2mA24yhc57tSZtb1kZUMuT7obdjFMRevD43U_zQyC54dBVMMP3YuzXqgZFzf578Iu6TrRoJoKsiJzTHRxJBSdP5cKQd9Oyv_sXhysth1g7N3utOgyD_U8L1c</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Madeira, Maria de Fátima</creator><creator>Schubach, Armando de Oliveira</creator><creator>Schubach, Tânia Maria Pacheco</creator><creator>Leal, Cristianni Antunes</creator><creator>Marzochi, Mauro Célio de Almeida</creator><general>Brazilian Society of Infectious Diseases</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20041201</creationdate><title>Identification of Leishmania (Leishmania) chagasi isolated from healthy skin of symptomatic and asymptomatic dogs seropositive for leishmaniasis in the municipality of Rio de Janeiro, Brazil</title><author>Madeira, Maria de Fátima ; 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subjects | Animals Brazil clinical signs dog Dog Diseases - parasitology Dogs Electrophoresis - methods Fluorescent Antibody Technique, Indirect INFECTIOUS DISEASES intact skin isoenzymes Isoenzymes - analysis Leishmania (Leishmania) chagasi Leishmania infantum - isolation & purification Leishmaniasis, Visceral - parasitology Leishmaniasis, Visceral - veterinary |
title | Identification of Leishmania (Leishmania) chagasi isolated from healthy skin of symptomatic and asymptomatic dogs seropositive for leishmaniasis in the municipality of Rio de Janeiro, Brazil |
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