A "Green" Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in Croton Crassifolius

Clerodane diterpenoids are the main bioactive constituents of and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) pr...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-02, Vol.24 (4), p.694
Main Authors: Tian, Jin-Long, Shu, Chi, Zhang, Ye, Cui, Hui-Jun, Xie, Xu, Ran, Xu-Long, Chen, Tian-Shun, Zang, Zhi-Huan, Liu, Jian-Guo, Li, Bin
Format: Article
Language:English
Subjects:
Acids
Chinese medicine
Chromatography
clerodane diterpenoids
Croton crassifolius
Diterpenes
Elution
Genetic analysis
Genetic diversity
homogenate extraction
Methods
Optimization
Polarity
Pretreatment
Principal components analysis
principle component analysis
Quality control
Quantitative analysis
Solvents
Spectrum analysis
Storage conditions
UHPLC–MS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Clerodane diterpenoids are the main bioactive constituents of and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) process combined with a sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC⁻MS) technique together for the rapid extraction and determination of clerodane diterpenoids in . All calibration curves showed good linearity ( > 0.9943) within the test ranges and the intra- and inter-day precisions and repeatability were all within required limits. This modified HGE⁻UHPLC⁻MS method only took 5 min to extract nine clerodane diterpenoids in and another 12 min to quantify these components. The results indicated that the quantitative analysis based on UHPLC⁻MS was a feasible method for QC of clerodane diterpenoids in , and the findings outlined in the current study also inferred the potential of the method in the QC of clerodane diterpenoids in other complex species of plants.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24040694