CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation

Dendritic cells (DCs) initiate immune responses through their direct interaction with effector cells. However, the mechanism by which DC activity is regulated is not well defined. Previous studies have shown that CTLA4 on T cells regulates DCs function by "cross-talk". We investigated whet...

Full description

Saved in:
Bibliographic Details
Published in:BMC immunology 2011-03, Vol.12 (1), p.21-21, Article 21
Main Authors: Wang, Xiong B, Fan, Zhong Z, Anton, Doina, Vollenhoven, Annika V, Ni, Zhen H, Chen, Xiao F, Lefvert, Ann K
Format: Article
Language:English
Subjects:
Antigen Presentation - immunology
Antigens
Antigens, CD - genetics
Antigens, CD - immunology
Antigens, CD - metabolism
Antigens, CD1 - immunology
Antigens, CD1 - metabolism
Arthritis
Cell Differentiation - immunology
Cells, Cultured
Chinese medicine
CTLA-4 Antigen
Dendritic cells
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - metabolism
Endocytosis - immunology
Flow Cytometry
Gene Expression
Genetic engineering
Humans
Immune response
Immunoglobulins
Life sciences
Ligands
Lymphocyte Culture Test, Mixed
Monocytes - cytology
Monocytes - immunology
Monocytes - metabolism
Physiological aspects
Reverse Transcriptase Polymerase Chain Reaction
Stem cells
T cells
Thoracic surgery
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dendritic cells (DCs) initiate immune responses through their direct interaction with effector cells. However, the mechanism by which DC activity is regulated is not well defined. Previous studies have shown that CTLA4 on T cells regulates DCs function by "cross-talk". We investigated whether there is an intrinsic regulatory mechanism in DCs, with CTLA4 as a candidate regulator. We confirmed via RT-PCR and flow cytometry the natural expression of CTLA4 on mature DCs derived from human monocytes. Approximately 8% CD1a-positive cells express CTLA4 both on surface and intracellular, whereas 10% CD1a-negative cells express CTLA4 intracellularly, but little expression was observed on the cell surface. The cross-linking of CTLA4 inhibits DCs maturation and antigen presentation in vitro, but does not inhibit endocytosis. CTLA4 is expressed by DCs and plays an inhibitory role. CTLA4-expressing DCs may represent a group of regulatory DCs. Because of its wide distribution on different cell types, CTLA4 may play a general role in regulating immune responses.
ISSN:1471-2172
1471-2172
DOI:10.1186/1471-2172-12-21