The Role of 20-HETE, COX, Thromboxane Receptors, and Blood Plasma Antioxidant Status in Vascular Relaxation of Copper-Nanoparticle-Fed WKY Rats

• Published in: Nutrients 2021-10, Vol.13 (11), p.3793
• Main Authors: Majewski, Michał, Juśkiewicz, Jerzy, Krajewska-Włodarczyk, Magdalena, Gromadziński, Leszek, Socha, Katarzyna, Cholewińska, Ewelina, Ognik, Katarzyna
• Format: Article
• Language: English
• Subjects: 20-HETE; aging; Animal models; Animals; Antioxidants; Antioxidants - metabolism; Aorta; Aorta, Thoracic - drug effects; Blood plasma; Catalase; Copper; Copper - administration & dosage; Coronary vessels; COX-2 inhibitors; Cyclooxygenase-1; Cyclooxygenase-2; Diet; Dietary intake; Dietary Supplements; Drug dosages; Endothelium, Vascular - drug effects; Enzymes; Feeds; furegrelate; HET0016; Hydroxyeicosatetraenoic Acids - blood; indomethacin; Male; Metal Nanoparticles - administration & dosage; Models, Animal; Nanoparticles; NS-398; Plasma; Prostaglandin-Endoperoxide Synthases - blood; Prostaglandins; Rats; Rats, Inbred WKY; Reactive oxygen species; Receptors, Thromboxane - blood; Superoxide dismutase; Thorax; Vasoconstriction - drug effects; Vasodilation; Vasodilation - drug effects; Vasodilator Agents - administration & dosage; Zinc
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13113793

Recently, the addition of copper nanoparticles (NPs) in a daily diet (6.5 mg/kg) was studied in different animal models as a possible alternative to ionic forms. Male Wistar-Kyoto rats (24-week-old, = 11) were fed with copper, either in the form of carbonate salt (Cu ) or metal-based copper NPs (NP ), for 8 weeks. The third group was fed with a half dose of each (NP + Cu ). The thoracic aorta and blood plasma was studied. Supplementation with NP decreased the Cu (×0.7), Cu/Zn-ratio (×0.6) and catalase (CAT, ×0.7), and increased Zn (×1.2) and superoxide dismutase (SOD, ×1.4). Meanwhile, NP + Cu decreased the Cu/Zn-ratio (×0.7), and CAT (×0.7), and increased the daily feed intake (×1.06). Preincubation with either the selective cyclooxygenase (COX)-2 inhibitor, or the non-selective COX-1/2 inhibitor attenuated vasodilation of rat thoracic aorta in the NP group exclusively. However, an increased vasodilator response was observed in the NP and NP + Cu group of rats after preincubation with an inhibitor of 20-hydroxyeicosatetraenoic acid (20-HETE) formation, and the thromboxane receptor (TP) antagonist. Significant differences were observed between the NP and NP + Cu groups of rats in: dietary intake, acetylcholine-induced vasodilation, and response to COX-inhibitors. Copper NPs in a standard daily dose had more significant effects on the mechanism(s) responsible for the utilization of reactive oxygen species in the blood plasma with the participation of prostanoids derived from COX-2 in the vascular relaxation. Dietary copper NPs in both doses modified vasodilation through the vasoconstrictor 20-HETE and the TP receptors.