Characterization of the growth-related transcriptome in the Liver and Brain of mandarin fish (Siniperca chuatsi) through RNA-Seq analysis

The brain and liver of mandarin fish (Siniperca chuatsi) produce essential hormones for their growth and development. However, the mechanisms underlying the interplay of these hormones remain unclear. This study aimed to assess the slow - and fast-growing traits, including body weight and length of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Applied Animal Research 2024-12, Vol.52 (1)
Main Authors: Ding, Weidong, Cao, Liping, Cao, Zheming, Bing, Xuwen
Format: Article
Language:English
Subjects:
Body weight
DEG
Fasting
Genes
growth
Growth hormones
Hormones
Insulin
Insulin-like growth factor-binding protein 3
Insulin-like growth factors
Kinases
Liver
Mandarin fish
MAP kinase
Sequence analysis
Siniperca chuatsi
Smad protein
transcriptome
Transcriptomes
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The brain and liver of mandarin fish (Siniperca chuatsi) produce essential hormones for their growth and development. However, the mechanisms underlying the interplay of these hormones remain unclear. This study aimed to assess the slow - and fast-growing traits, including body weight and length of mandarin fish reared in a single pond. To compare the differentially expressed genes (DEGs) in slow - and fast-growing fishes, we performed RNA sequencing analysis. After assembling the clean reads, 192,280 transcripts and 100,416 unigenes were obtained. 344 and 845 DEGs in the brain and liver in the fast-and slow-growing group were annotated respectively. These DEGs were associated with signaling pathways involved in growth and development, such as AGE-RAGE, Transforming growth factor-β/Smad, Mitogen-activated protein kinase, Wingless/Integrated, and Insulin-like growth factor (IGF) 1. Furthermore, our fasting experiment revealed a concurrent decrease in body weight and the expression levels of genes related to the growth hormone (GH)/IGF system, alongside an increase in the insulin-like growth factor binding protein gene IGFBP3 expression. These findings suggest the GH/IGF system regulates body weight during fasting periods. The vast amount of transcriptome data generated in this study substantially expands the existing gene and genome resources for the growth traits of mandarin fish.
ISSN:0971-2119
0974-1844
DOI:10.1080/09712119.2024.2440045