Simultaneous Application of Several Exogenous dsRNAs for the Regulation of Anthocyanin Biosynthesis in Arabidopsis thaliana

Plant surface treatment with double-stranded RNAs (dsRNAs) has gained recognition as a promising method for inducing gene silencing and combating plant pathogens. However, the regulation of endogenous plant genes by external dsRNAs has not been sufficiently investigated. Also, the effect of the simu...

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Bibliographic Details
Published in:Plants (Basel) 2024-02, Vol.13 (4), p.541
Main Authors: Kiselev, Konstantin V, Suprun, Andrey R, Aleynova, Olga A, Ogneva, Zlata V, Dubrovina, Alexandra S
Format: Article
Language:English
Subjects:
Accumulation
Anthocyanidin reductase
anthocyanin biosynthesis
Anthocyanins
Aqueous solutions
Arabidopsis thaliana
Biosynthesis
Biotechnology
Calcium-binding protein
Calmodulin
Chalcone synthase
dsRNA foliar application
dsRNA plant surface treatment
Foliar applications
Gene expression
Gene regulation
Gene silencing
Genes
Genomes
Liquid chromatography
Pathogens
Reductases
RNA polymerase
RNAi
Surface treatment
Transcription factors
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Summary:Plant surface treatment with double-stranded RNAs (dsRNAs) has gained recognition as a promising method for inducing gene silencing and combating plant pathogens. However, the regulation of endogenous plant genes by external dsRNAs has not been sufficiently investigated. Also, the effect of the simultaneous application of multiple gene-specific dsRNAs has not been analyzed. The aim of this study was to exogenously target five genes in , namely, three transcription factor genes ( , , ), a calmodulin-binding protein gene ( ), and an anthocyanidin reductase gene ( ), which are known as negative regulators of anthocyanin accumulation. Exogenous dsRNAs encoding these genes were applied to the leaf surface of either individually or in mixtures. The mRNA levels of the five targets were analyzed using qRT-PCR, and anthocyanin content was evaluated through HPLC-MS. The results demonstrated significant downregulation of all five target genes by the exogenous dsRNAs, resulting in enhanced expression of chalcone synthase ( ) gene and increased anthocyanin content. The simultaneous foliar application of the five dsRNAs proved to be more efficient in activating anthocyanin accumulation compared to the application of individual dsRNAs. These findings hold considerable importance in plant biotechnology and gene function studies.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13040541