Tartrazine Modifies the Activity of DNMT and HDAC Genes-Is This a Link between Cancer and Neurological Disorders?

In recent years, artificial additives, especially synthetic food colorants, were found to demonstrate wider properties compared to their natural equivalents; however, their health impact is still not totally mapped. Our study aimed to determine the long-term (30 and 90 days) exposure effect of one o...

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Bibliographic Details
Published in:Nutrients 2023-06, Vol.15 (13), p.2946
Main Authors: Zand, Afshin, Enkhbilguun, Sodbuyan, Macharia, John M, Budán, Ferenc, Gyöngyi, Zoltán, Varjas, Timea
Format: Article
Language:English
Subjects:
Acids
additive
Additives
Animals
Asthma
Biopsy
Cancer
Carcinogenesis
Carcinogens
Cell cycle
Cell growth
Coloring agents
DNA methylation
DNA methyltransferase
DNMT
Drinking water
Dyes
Enzymes
Epigenetics
Exposure
Females
Food
Food additives
Food Coloring Agents - adverse effects
Food Coloring Agents - analysis
Food dyes
Gene expression
Genes
HDAC
Histone deacetylase
Histone Deacetylases - genetics
Histone Deacetylases - metabolism
Humans
Mice
Mice, Inbred Strains
Neoplasms - genetics
Nervous System Diseases
Neurological diseases
Neurological disorders
qRT-PCR
Reverse transcription
Rodents
Tartrazine
Tartrazine - analysis
Temperature
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Summary:In recent years, artificial additives, especially synthetic food colorants, were found to demonstrate wider properties compared to their natural equivalents; however, their health impact is still not totally mapped. Our study aimed to determine the long-term (30 and 90 days) exposure effect of one of the commonly used artificial food colorants, tartrazine, on NMRI mice. The applied dose of tartrazine referred to the human equivalent dose for acceptable daily intake (ADI). Further, we evaluated its impact on the transcription of a range of epigenetic effectors, members of the DNA methyltransferase ( ) as well as histone deacetylase ( families. Following the exposure, organ biopsies were collected from the lungs, kidneys, liver, and spleen, and the gene expression levels were determined by real-time quantitative reverse transcription PCR (RT-qPCR). Our results demonstrated significant upregulation of genes in the tested organs in various patterns followed by the intake of tartrazine on ADI. Since and genes are involved in different steps of carcinogenesis, have roles in the development of neurological disorders and the effect of dose of everyday exposure is rarely studied, further investigation is warranted to study these possible associations.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu15132946