Molecular record for the first authentication of Isaria cicadae from Vietnam

• Published in: Open life sciences 2021-07, Vol.16 (1), p.711-718
• Main Authors: Lao, Thuan Duc, Van Trinh, Hanh, Vuong, Loi, Tien Vu, Luyen, Huyen Le, Thuy Ai, Dinh, Hiep Minh, Truong, Nguyen Binh
• Format: Article
• Language: English
• Subjects: isaria cicadae; nuclear large ribosomal subunit; nuclear small ribosomal subunit; phylogeny
• ISSN: 2391-5412; 2391-5412
• DOI: 10.1515/biol-2021-0074

The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as , belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including , , , , and was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify , , , , and gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus . The phylogenetic analysis results indicated that T011 was accepted at subclade and significantly formed the monophyletic group with referent (Telemorph of ) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the . In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as