The effect of carbonic anhydrase on foraminiferal Mg/Ca

• Published in: PeerJ (San Francisco, CA) CA), 2024-11, Vol.12, p.e18458, Article e18458
• Main Authors: De Goeyse, Siham, Lesuis, Chiara, Reichart, Gert-Jan, de Nooijer, Lennart
• Format: Article
• Language: English
• Subjects: Acetazolamide; Acetazolamide - pharmacology; Aquatic and Marine Chemistry; Biogeochemistry; Biological Oceanography; Calcification; Calcification, Physiologic - drug effects; Calcite; Calcium - metabolism; Calcium carbonate; Calcium Carbonate - metabolism; Calcium influx; Carbon; Carbon dioxide; Carbon Dioxide - metabolism; Carbonic Anhydrase Inhibitors - chemistry; Carbonic Anhydrase Inhibitors - pharmacology; Carbonic anhydrases; Carbonic Anhydrases - metabolism; Enzymes; Foraminifera; Foraminifera - metabolism; Geochemistry; Inorganic carbon; Magnesium - metabolism; Paleoceanography; Precipitation; Seawater; Sediments; Shells
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.18458

Marine biogenic calcium carbonate production plays a role in the exchange of CO between ocean and atmosphere. The effect of increased CO on calcification and on the resulting chemistry of shells and skeletons, however, is only partly understood. Foraminifera are among the main marine CaCO producers and the controls on element partitioning and isotope fractionation is the subject of many recent investigations. The enzyme carbonic anhydrase (CA) was, for example, shown to be vital for CaCO deposition in benthic foraminifera and indicates their ability to manipulate their intracellular inorganic carbon chemistry. Here, we tested whether CA affects the partitioning of Na, Mg and Sr in the perforate, large benthic, symbiont-bearing foraminifer by addition of the inhibitor acetazolamide (AZ). The effect of dissolved CO on the effect of CA on element partitioning was also determined using a culturing setup with controlled atmospheric carbon dioxide levels (400-1,600 ppm). Results show that inhibition by AZ reduces calcification greatly and that CO has a small, but positive effect on the amount of calcite formed during the incubations. Furthermore, the inhibition of CA activity has a positive effect on element partitioning, most notably Mg. This may be explained by a (n indirect) coupling of inorganic carbon uptake and inward calcium ion pumping.