Inhibitory effects of vaginal Lactobacilli on Candida albicans growth, hyphal formation, biofilm development, and epithelial cell adhesion

• Published in: Frontiers in cellular and infection microbiology 2023-05, Vol.13, p.1113401-1113401
• Main Authors: Takano, Tomonori, Kudo, Hayami, Eguchi, Shuhei, Matsumoto, Asami, Oka, Kentaro, Yamasaki, Yukitaka, Takahashi, Motomichi, Koshikawa, Takuro, Takemura, Hiromu, Yamagishi, Yuka, Mikamo, Hiroshige, Kunishima, Hiroyuki
• Format: Article
• Language: English
• Subjects: biofilm; Candida albicans; cell adhesion; Cellular and Infection Microbiology; Lactobacillus species; probiotics
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2023.1113401

IntroductionAntifungal agents are not always efficient in resolving vulvovaginal candidiasis (VVC), a common genital infection caused by the overgrowth of Candida spp., including Candida albicans, or in preventing recurrent infections. Although lactobacilli (which are dominant microorganisms constituting healthy human vaginal microbiota) are important barriers against VVC, the Lactobacillus metabolite concentration needed to suppress VVC is unknown. MethodsWe quantitatively evaluated Lactobacillus metabolite concentrations to determine their effect on Candida spp., including 27 vaginal strains of Lactobacillus crispatus, L. jensenii, L. gasseri, Lacticaseibacillus rhamnosus, and Limosilactobacillus vaginalis, with inhibitory abilities against biofilms of C. albicans clinical isolates. ResultsLactobacillus culture supernatants suppressed viable fungi by approximately 24%-92% relative to preformed C. albicans biofilms; however, their suppression differed among strains and not species. A moderate negative correlation was found between Lactobacillus lactate production and biofilm formation, but no correlation was observed between hydrogen peroxide production and biofilm formation. Both lactate and hydrogen peroxide were required to suppress C. albicans planktonic cell growth. Lactobacillus strains that significantly inhibited biofilm formation in culture supernatant also inhibited C. albicans adhesion to epithelial cells in an actual live bacterial adhesion competition test. DiscussionHealthy human microflora and their metabolites may play important roles in the development of new antifungal agent against C. albicans-induced VVC.