Comprehensive Metabolite Profiling of Chemlali Olive Tree Root Extracts Using LC-ESI-QTOF-MS/MS, Their Cytotoxicity, and Antiviral Assessment

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-06, Vol.28 (12), p.4829
• Main Authors: Toumi, Karim, Świątek, Łukasz, Boguszewska, Anastazja, Skalicka-Woźniak, Krystyna, Bouaziz, Mohamed
• Format: Article
• Language: English
• Subjects: Acids; Animals; Antiviral activity; Antiviral agents; biological activities; By products; Chlorocebus aethiops; Chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Liquid; Coxsackievirus infections; Cultivars; Cytotoxicity; Flavonoids; Flavonoids - chemistry; Fruit trees; Herbal medicine; Herpes viruses; Humans; Instrument industry; Iridoids - chemistry; LC-MS; Lignans; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolites; Olea - chemistry; olive roots; Organic acids; Oxidation; Phenolic acids; phenolic compounds; Phenols; Phenols - chemistry; Physiology; Phytochemicals; Plant Extracts - chemistry; Plant Leaves - chemistry; Roots; Scientific imaging; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; Trees; ultrasonic extraction; Vero Cells
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28124829

The large quantity of olive roots resulting from a large number of old and unfruitful trees encouraged us to look for ways of adding value to these roots. For this reason, the current research work is devoted to the valorization of olive roots by identifying active phytochemicals and assessing their biological activities, including the cytotoxicity and antiviral potential of different extracts from the Chemlali cultivar. The extract, obtained by ultrasonic extraction, was analyzed using the liquid chromatography-mass spectrometry technique (LC-MS). The cytotoxicity was evaluated through the use of the microculture tetrazolium assay (MTT) against VERO cells. Subsequently, the antiviral activity was determined for HHV-1 (Human Herpesvirus type 1) and CVB3 (Coxsackievirus B3) replication in the infected VERO cells. LC-MS analysis allowed the identification of 40 compounds, classified as secoiridoids (53%), organic acids (13%), iridoids (10%), lignans (8%), caffeoylphenylethanoid (5%), phenylethanoids (5%),sugars and derivatives (2%), phenolic acids (2%), and flavonoids (2%). It was found that extracts were not toxic to the VERO cells. Moreover, the extracts did not influence the appearance of HHV-1 or CVB3 cytopathic effects in the infected VERO cells and failed to decrease the viral infectious titer.