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Cloning and functional characterization of the legumin A gene (EuLEGA) from Eucommia ulmoides Oliver
Legumin A is a seed storage protein that provides nutrients for seed germination. The purpose of this study was to describe the structure and expression pattern of the EuLEGA gene in Eucommia ulmoides Oliver ( E. ulmoides ) and to infer its functional role. The 1287 bp coding sequence of the EuLEGA...
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description | Legumin A is a seed storage protein that provides nutrients for seed germination. The purpose of this study was to describe the structure and expression pattern of the
EuLEGA
gene in
Eucommia ulmoides
Oliver (
E. ulmoides
) and to infer its functional role. The 1287 bp coding sequence of the
EuLEGA
CDS of the
EuLEGA
gene, encoding a protein containing 428 amino acid residues, was cloned. The structure predicted that the protein belonged to the RmlC (deoxythymidine diphosphates, dTDP)-4-dehydrorhamnose 3,5-epimerase)-like cupin conserved domain family, which contains both RmlC, a key enzyme for the synthesis of rhamnose and legumin A. The overexpression (OE) vector of the
EuLEGA
gene was constructed and genetically transformed into tobacco and
E. ulmoides
; the RNA interference (RNAi) vector of the
EuLEGA
gene was constructed and genetically transformed into
E. ulmoides
; and the contents of legumin A and rhamnose were detected. The results showed that the
EuLEGA
gene could significantly increase the content of legumin A in transgenic tobacco leaves and transgenic
E. ulmoides
regenerative buds, and the OE of this gene in
E. ulmoides
could promote an increase in rhamnose content. RNAi caused a significant decrease in the legumin A content in the regenerated buds of
E. ulmoides
. These was a significant increase in legumin A in the transgenic tobacco seeds, and these results indicate that the expression of the
EuLEGA
gene is closely related to the accumulation of legumin A. Subcellular localization studies revealed that EuLEGA is localized to the cytoplasm with the vacuolar membrane. Analysis of the
EuLEGA
gene expression data revealed that the expression level of the
EuLEGA
gene in the samaras was significantly greater than that in the leaves and stems. In addition, the study also demonstrated that GA
3
can upregulate the expression levels of the
EuLEGA
gene, while ABA and MeJA can downregulate its expression levels. |
doi_str_mv | 10.1038/s41598-024-65020-5 |
format | article |
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EuLEGA
gene in
Eucommia ulmoides
Oliver (
E. ulmoides
) and to infer its functional role. The 1287 bp coding sequence of the
EuLEGA
CDS of the
EuLEGA
gene, encoding a protein containing 428 amino acid residues, was cloned. The structure predicted that the protein belonged to the RmlC (deoxythymidine diphosphates, dTDP)-4-dehydrorhamnose 3,5-epimerase)-like cupin conserved domain family, which contains both RmlC, a key enzyme for the synthesis of rhamnose and legumin A. The overexpression (OE) vector of the
EuLEGA
gene was constructed and genetically transformed into tobacco and
E. ulmoides
; the RNA interference (RNAi) vector of the
EuLEGA
gene was constructed and genetically transformed into
E. ulmoides
; and the contents of legumin A and rhamnose were detected. The results showed that the
EuLEGA
gene could significantly increase the content of legumin A in transgenic tobacco leaves and transgenic
E. ulmoides
regenerative buds, and the OE of this gene in
E. ulmoides
could promote an increase in rhamnose content. RNAi caused a significant decrease in the legumin A content in the regenerated buds of
E. ulmoides
. These was a significant increase in legumin A in the transgenic tobacco seeds, and these results indicate that the expression of the
EuLEGA
gene is closely related to the accumulation of legumin A. Subcellular localization studies revealed that EuLEGA is localized to the cytoplasm with the vacuolar membrane. Analysis of the
EuLEGA
gene expression data revealed that the expression level of the
EuLEGA
gene in the samaras was significantly greater than that in the leaves and stems. In addition, the study also demonstrated that GA
3
can upregulate the expression levels of the
EuLEGA
gene, while ABA and MeJA can downregulate its expression levels.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-024-65020-5</identifier><identifier>PMID: 38898092</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647 ; 631/337 ; 631/449 ; 631/45 ; 631/61 ; 631/80 ; 631/92 ; Abscisic acid ; Amino acids ; Buds ; Cloning ; Cloning, Molecular ; Cytoplasm ; Epimerase ; Eucommia ulmoides ; Eucommiaceae - genetics ; Eucommiaceae - metabolism ; Gene expression ; Gene Expression Regulation, Plant ; Humanities and Social Sciences ; Leaves ; Legumin ; Legumin A ; Legumin A-encoding gene ; Legumins - genetics ; Legumins - metabolism ; Localization ; multidisciplinary ; Nicotiana - genetics ; Nicotiana - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Protein structure ; Proteins ; Rhamnose ; Rhamnose - metabolism ; RNA Interference ; RNA-mediated interference ; Science ; Science (multidisciplinary) ; Seed germination ; Tobacco ; Transgene ; Transgenic plants</subject><ispartof>Scientific reports, 2024-06, Vol.14 (1), p.14111-16, Article 14111</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c422t-a213f4b0fac804e058c2bc83dee0fb8c74b642cd26d8a9ab62d7b0b674c77e1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3069699906/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3069699906?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38898092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Lina</creatorcontrib><creatorcontrib>Zhao, De-Gang</creatorcontrib><title>Cloning and functional characterization of the legumin A gene (EuLEGA) from Eucommia ulmoides Oliver</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Legumin A is a seed storage protein that provides nutrients for seed germination. The purpose of this study was to describe the structure and expression pattern of the
EuLEGA
gene in
Eucommia ulmoides
Oliver (
E. ulmoides
) and to infer its functional role. The 1287 bp coding sequence of the
EuLEGA
CDS of the
EuLEGA
gene, encoding a protein containing 428 amino acid residues, was cloned. The structure predicted that the protein belonged to the RmlC (deoxythymidine diphosphates, dTDP)-4-dehydrorhamnose 3,5-epimerase)-like cupin conserved domain family, which contains both RmlC, a key enzyme for the synthesis of rhamnose and legumin A. The overexpression (OE) vector of the
EuLEGA
gene was constructed and genetically transformed into tobacco and
E. ulmoides
; the RNA interference (RNAi) vector of the
EuLEGA
gene was constructed and genetically transformed into
E. ulmoides
; and the contents of legumin A and rhamnose were detected. The results showed that the
EuLEGA
gene could significantly increase the content of legumin A in transgenic tobacco leaves and transgenic
E. ulmoides
regenerative buds, and the OE of this gene in
E. ulmoides
could promote an increase in rhamnose content. RNAi caused a significant decrease in the legumin A content in the regenerated buds of
E. ulmoides
. These was a significant increase in legumin A in the transgenic tobacco seeds, and these results indicate that the expression of the
EuLEGA
gene is closely related to the accumulation of legumin A. Subcellular localization studies revealed that EuLEGA is localized to the cytoplasm with the vacuolar membrane. Analysis of the
EuLEGA
gene expression data revealed that the expression level of the
EuLEGA
gene in the samaras was significantly greater than that in the leaves and stems. In addition, the study also demonstrated that GA
3
can upregulate the expression levels of the
EuLEGA
gene, while ABA and MeJA can downregulate its expression levels.</description><subject>631/1647</subject><subject>631/337</subject><subject>631/449</subject><subject>631/45</subject><subject>631/61</subject><subject>631/80</subject><subject>631/92</subject><subject>Abscisic acid</subject><subject>Amino acids</subject><subject>Buds</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Cytoplasm</subject><subject>Epimerase</subject><subject>Eucommia ulmoides</subject><subject>Eucommiaceae - genetics</subject><subject>Eucommiaceae - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Humanities and Social Sciences</subject><subject>Leaves</subject><subject>Legumin</subject><subject>Legumin A</subject><subject>Legumin A-encoding gene</subject><subject>Legumins - genetics</subject><subject>Legumins - metabolism</subject><subject>Localization</subject><subject>multidisciplinary</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Protein structure</subject><subject>Proteins</subject><subject>Rhamnose</subject><subject>Rhamnose - metabolism</subject><subject>RNA Interference</subject><subject>RNA-mediated interference</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Seed germination</subject><subject>Tobacco</subject><subject>Transgene</subject><subject>Transgenic plants</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kktv3CAUha2qVROl-QNdVEjdpAungLENq2o0mqaRRsqmXSMeFw8jG1KwI7W_vkwmzaOLsgHde-53QJyqek_wJcEN_5wZaQWvMWV112KK6_ZVdUoxa2vaUPr62fmkOs95j8tqqWBEvK1OGs4Fx4KeVnY9xuDDgFSwyC3BzD4GNSKzU0mZGZL_rQ4lFB2ad4BGGJbJB7RCAwRAF5tlu7lafUIuxQltFhOnySu0jFP0FjK6Gf0dpHfVG6fGDOcP-1n14-vm-_pbvb25ul6vtrVhlM61oqRxTGOnDMcMcMsN1YY3FgA7zU3PdMeosbSzXAmlO2p7jXXXM9P3QKA5q66PXBvVXt4mP6n0S0bl5X0hpkGqNHszgnRKE17sTKcJs8JysMS44otbZ6CjhfXlyLpd9ATWQJiTGl9AX3aC38kh3klCCO9J0xfCxQMhxZ8L5FlOPhsYRxUgLlk2uMectpiJIv34j3Qfl1T-4aDqRCeEwF1R0aPKpJhzAvd4G4LlIRTyGApZQiHvQyHbMvTh-TseR_5GoAiaoyCXVhggPXn_B_sHsqXDIQ</recordid><startdate>20240619</startdate><enddate>20240619</enddate><creator>Zheng, Lina</creator><creator>Zhao, De-Gang</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20240619</creationdate><title>Cloning and functional characterization of the legumin A gene (EuLEGA) from Eucommia ulmoides Oliver</title><author>Zheng, Lina ; Zhao, De-Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-a213f4b0fac804e058c2bc83dee0fb8c74b642cd26d8a9ab62d7b0b674c77e1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>631/1647</topic><topic>631/337</topic><topic>631/449</topic><topic>631/45</topic><topic>631/61</topic><topic>631/80</topic><topic>631/92</topic><topic>Abscisic acid</topic><topic>Amino acids</topic><topic>Buds</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Cytoplasm</topic><topic>Epimerase</topic><topic>Eucommia ulmoides</topic><topic>Eucommiaceae - genetics</topic><topic>Eucommiaceae - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Humanities and Social Sciences</topic><topic>Leaves</topic><topic>Legumin</topic><topic>Legumin A</topic><topic>Legumin A-encoding gene</topic><topic>Legumins - genetics</topic><topic>Legumins - metabolism</topic><topic>Localization</topic><topic>multidisciplinary</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Protein structure</topic><topic>Proteins</topic><topic>Rhamnose</topic><topic>Rhamnose - metabolism</topic><topic>RNA Interference</topic><topic>RNA-mediated interference</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Seed germination</topic><topic>Tobacco</topic><topic>Transgene</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Lina</creatorcontrib><creatorcontrib>Zhao, De-Gang</creatorcontrib><collection>Springer_OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest Publicly Available Content database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Lina</au><au>Zhao, De-Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and functional characterization of the legumin A gene (EuLEGA) from Eucommia ulmoides Oliver</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2024-06-19</date><risdate>2024</risdate><volume>14</volume><issue>1</issue><spage>14111</spage><epage>16</epage><pages>14111-16</pages><artnum>14111</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Legumin A is a seed storage protein that provides nutrients for seed germination. The purpose of this study was to describe the structure and expression pattern of the
EuLEGA
gene in
Eucommia ulmoides
Oliver (
E. ulmoides
) and to infer its functional role. The 1287 bp coding sequence of the
EuLEGA
CDS of the
EuLEGA
gene, encoding a protein containing 428 amino acid residues, was cloned. The structure predicted that the protein belonged to the RmlC (deoxythymidine diphosphates, dTDP)-4-dehydrorhamnose 3,5-epimerase)-like cupin conserved domain family, which contains both RmlC, a key enzyme for the synthesis of rhamnose and legumin A. The overexpression (OE) vector of the
EuLEGA
gene was constructed and genetically transformed into tobacco and
E. ulmoides
; the RNA interference (RNAi) vector of the
EuLEGA
gene was constructed and genetically transformed into
E. ulmoides
; and the contents of legumin A and rhamnose were detected. The results showed that the
EuLEGA
gene could significantly increase the content of legumin A in transgenic tobacco leaves and transgenic
E. ulmoides
regenerative buds, and the OE of this gene in
E. ulmoides
could promote an increase in rhamnose content. RNAi caused a significant decrease in the legumin A content in the regenerated buds of
E. ulmoides
. These was a significant increase in legumin A in the transgenic tobacco seeds, and these results indicate that the expression of the
EuLEGA
gene is closely related to the accumulation of legumin A. Subcellular localization studies revealed that EuLEGA is localized to the cytoplasm with the vacuolar membrane. Analysis of the
EuLEGA
gene expression data revealed that the expression level of the
EuLEGA
gene in the samaras was significantly greater than that in the leaves and stems. In addition, the study also demonstrated that GA
3
can upregulate the expression levels of the
EuLEGA
gene, while ABA and MeJA can downregulate its expression levels.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>38898092</pmid><doi>10.1038/s41598-024-65020-5</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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source | Open Access: PubMed Central; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access; ProQuest Publicly Available Content database |
subjects | 631/1647 631/337 631/449 631/45 631/61 631/80 631/92 Abscisic acid Amino acids Buds Cloning Cloning, Molecular Cytoplasm Epimerase Eucommia ulmoides Eucommiaceae - genetics Eucommiaceae - metabolism Gene expression Gene Expression Regulation, Plant Humanities and Social Sciences Leaves Legumin Legumin A Legumin A-encoding gene Legumins - genetics Legumins - metabolism Localization multidisciplinary Nicotiana - genetics Nicotiana - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Protein structure Proteins Rhamnose Rhamnose - metabolism RNA Interference RNA-mediated interference Science Science (multidisciplinary) Seed germination Tobacco Transgene Transgenic plants |
title | Cloning and functional characterization of the legumin A gene (EuLEGA) from Eucommia ulmoides Oliver |
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