Recombinant Toxoplasma gondii phosphoglycerate mutase 2 confers protective immunity against toxoplasmosis in BALB/c mice

Toxoplasmosis is one of the most widespread zoonoses worldwide. It has a high incidence and can result in severe disease in humans and livestock. Effective vaccines are needed to limit and prevent infection with Toxoplasma gondii. In this study, we evaluated the immuno-protective efficacy of a recom...

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Bibliographic Details
Published in:Parasite (Paris) 2016, Vol.23, p.12-12
Main Authors: Wang, Hai-Long, Wen, Li-Min, Pei, Yan-Jiang, Wang, Fen, Yin, Li-Tian, Bai, Ji-Zhong, Guo, Rui, Wang, Chun-Fang, Yin, Guo-Rong
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animal diseases
Animals
Antibodies
Brain - parasitology
Cell culture
Cells, Cultured
Chronic infection
Cytokines
Cytokines - blood
Disease control
Drug Evaluation
Effectiveness
Female
Immunity
Immunity, Mucosal
Immunization
Immunoglobulin A
Immunoglobulin A, Secretory - analysis
Immunoglobulin G
Immunoglobulin G - blood
Incidence
Infections
Interleukin 2
Interleukin 4
Liver - parasitology
Livestock
Lymphocyte Activation
Lymphocytes
Mice
Mice, Inbred BALB C
Mucosal immunity
Nasal vaccine
Peyer's Patches - immunology
Phosphoglycerate mutase
Phosphoglycerate Mutase - genetics
Phosphoglycerate Mutase - immunology
Phosphoglycerate mutase 2
Protozoa
Protozoan Proteins - genetics
Protozoan Proteins - immunology
Protozoan Vaccines - administration & dosage
Protozoan Vaccines - immunology
Recombinant protein
Recombinant Proteins - immunology
Rodents
Spleen - immunology
Survival
Tachyzoites
Toxoplasma - enzymology
Toxoplasma - genetics
Toxoplasma - growth & development
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis, Animal - blood
Toxoplasmosis, Animal - immunology
Toxoplasmosis, Animal - parasitology
Toxoplasmosis, Animal - prevention & control
Vaccination
Vaccines
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Zoonoses
γ-Interferon
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Summary:Toxoplasmosis is one of the most widespread zoonoses worldwide. It has a high incidence and can result in severe disease in humans and livestock. Effective vaccines are needed to limit and prevent infection with Toxoplasma gondii. In this study, we evaluated the immuno-protective efficacy of a recombinant Toxoplasma gondii phosphoglycerate mutase 2 (rTgPGAM 2) against T. gondii infection in BALB/c mice. We report that the mice nasally immunised with rTgPGAM 2 displayed significantly higher levels of special IgG antibodies against rTgPGAM 2 (including IgG1, IgG2a and IgAs) and cytokines (including IFN-γ, IL-2 and IL-4) in their blood sera and supernatant of cultured spleen cells compared to those of control animals. In addition, an increased number of spleen lymphocytes and enhanced lymphocyte proliferative responses were observed in the rTgPGAM 2-immunised mice. After chronic infection and lethal challenge with the highly virulent T. gondii RH strain by oral gavage, the survival time of the rTgPGAM 2-immunised mice was longer (P 
ISSN:1776-1042
1252-607X
1776-1042
DOI:10.1051/parasite/2016012