How Dopamine Influences Survival and Cellular Immune Response of Rhipicephalus microplus Inoculated with Metarhizium anisopliae

Dopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA’s role in the immune system of ticks. The use of Metarhizium anisopliae has been considered for tick control, driving studies on t...

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Published in:Journal of fungi (Basel) 2021-11, Vol.7 (11), p.950
Main Authors: Corrêa, Thaís Almeida, Fiorotti, Jéssica, Mesquita, Emily, Meirelles, Laura Nóbrega, Camargo, Mariana Guedes, Coutinho-Rodrigues, Caio Junior Balduino, Marciano, Allan Felipe, Bittencourt, Vânia Rita Elias Pinheiro, Golo, Patrícia Silva
Format: Article
Language:English
Subjects:
Arthropods
Biological control
Dopamine
Entomopathogenic fungi
Experiments
Fungi
Hemocytes
Hemolymph
Immune response
Immune response (cell-mediated)
Inoculation
Metarhizium anisopliae
Phagocytes
phagocytic activity
Phenoloxidase
Rhipicephalus microplus
ticks
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Summary:Dopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA’s role in the immune system of ticks. The use of Metarhizium anisopliae has been considered for tick control, driving studies on the immune response of these arthropods challenged with fungi. The present study evaluated the effect of DA on the cellular immune response and survival of Rhipicephalus microplus inoculated with M. anisopliae blastospores. Exogenous DA increased both ticks’ survival 72 h after M. anisopliae inoculation and the number of circulating hemocytes compared to the control group, 24 h after the treatment. The phagocytic index of tick hemocytes challenged with M. anisopliae did not change upon injection of exogenous DA. Phenoloxidase activity in the hemolymph of ticks injected with DA and the fungus or exclusively with DA was higher than in untreated ticks or ticks inoculated with the fungus alone, 72 h after treatment. DA was detected in the hemocytes of fungus-treated and untreated ticks. Unveiling the cellular immune response in ticks challenged with entomopathogenic fungi is important to improve strategies for the biological control of these ectoparasites.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof7110950