Dietary cis-9, trans-11-conjugated linoleic acid reduces amyloid β-protein accumulation and upregulates anti-inflammatory cytokines in an Alzheimer’s disease mouse model

• Published in: Scientific reports 2021-05, Vol.11 (1), p.9749-9749, Article 9749
• Main Authors: Fujita, Yu, Kano, Kuniyuki, Kishino, Shigenobu, Nagao, Toshihiro, Shen, Xuefeng, Sato, Chiharu, Hatakeyama, Hatsune, Ota, Yume, Niibori, Sho, Nomura, Ayako, Kikuchi, Kota, Yasuno, Wataru, Takatori, Sho, Kikuchi, Kazunori, Sano, Yoshitake, Tomita, Taisuke, Suzuki, Toshiharu, Aoki, Junken, Zou, Kun, Natori, Shunji, Komano, Hiroto
• Format: Article
• Language: English
• Subjects: 631/378; 631/80; 692/308; 692/420; 692/699; Alzheimer Disease - diet therapy; Alzheimer Disease - metabolism; Alzheimer Disease - pathology; Alzheimer's disease; Amyloid; Amyloid beta-Peptides - analysis; Amyloid beta-Peptides - metabolism; Animal models; Animals; Astrocytes; Cytokines; Cytokines - analysis; Cytokines - metabolism; Diet; Dietary Fats, Unsaturated - therapeutic use; Dietary intake; Disease Models, Animal; Humanities and Social Sciences; Humans; Inflammation; Interleukin 10; Interleukin 19; Linoleic acid; Linoleic Acids, Conjugated - therapeutic use; Liquid chromatography; Lysophosphatidylcholine; Mass spectrometry; Mass spectroscopy; Mice; Mice, Inbred C57BL; Microglia; multidisciplinary; Neurodegenerative diseases; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-88870-9

Conjugated linoleic acid (CLA) is an isomer of linoleic acid (LA). The predominant dietary CLA is cis -9, trans -11-CLA ( c -9, t -11-CLA), which constitutes up to ~ 90% of total CLA and is thought to be responsible for the positive health benefits associated with CLA. However, the effects of c -9, t -11-CLA on Alzheimer’s disease (AD) remain to be elucidated. In this study, we investigated the effect of dietary intake of c -9, t -11-CLA on the pathogenesis of an AD mouse model. We found that c -9, t -11-CLA diet-fed AD model mice significantly exhibited (1) a decrease in amyloid-β protein (Aβ) levels in the hippocampus, (2) an increase in the number of microglia, and (3) an increase in the number of astrocytes expressing the anti-inflammatory cytokines, interleukin-10 and 19 (IL-10, IL-19), with no change in the total number of astrocytes. In addition, liquid chromatography–tandem mass spectrometry (LC–MS/MS) and gas chromatographic analysis revealed that the levels of lysophosphatidylcholine (LPC) containing c -9, t -11-CLA (CLA-LPC) and free c -9, t -11-CLA were significantly increased in the brain of c -9, t -11-CLA diet-fed mice. Thus, dietary c -9, t -11-CLA entered the brain and appeared to exhibit beneficial effects on AD, including a decrease in Aβ levels and suppression of inflammation.